FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528707
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1228884.859716843430259No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA242460.9588299474790871No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC210740.8333903453425011No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT167770.6634616031038788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163920.6482364307134041No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA152550.6032727397836127No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG145340.5747601442159965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137900.5453379928951831No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC133240.5269096024173618No Hit
CAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA132030.5221245482374984No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT116580.46102612916403524No Hit
GTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATAATGATGT113370.4483318945215875No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC106370.42064976290254275No Hit
GACTAAGGCTATCTGCTTCCCTTATGCCCTGACTGGAGAGGGGAGGGAGG101850.4027750150571023No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA99990.39541947722689896No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA89330.353263545361325No Hit
GAATAATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGG83630.3307223810429599No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC82660.32688642851860655No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC78380.3099607823286763No Hit
ATGCTGCCCAGTGGCTTCCGGAAGTTCCTGGTCCACAACGTCAAGGAGCT78020.3085371298454111No Hit
CAGGAGGCGCTGCGCCTGCAGGCCGCGCTGGAGATGCGGGCGGCCGACGC76570.30280297401003753No Hit
GAACAACTACTCTCTTCAAAGCTGTCAGACAGAGACATTTAAGTCTGCAG75370.298057465732487No Hit
TTTTGTTTATGAGCAAGGTGGGTCTCAGAGGTGATCGGCGATCAGAGGGC73440.2904251065860932No Hit
TGTCTGGAGGCAGAAAACACCCCTCTTTGTCTTTGTAAATGGAGTGGTTG70510.2788381572084073No Hit
CACTGTAACCTCCACTTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTC69620.2753185719025573No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG69550.2750417505863668No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA68980.27278763415453033No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG67250.265946193054395No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC62220.24605460419099565No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57010.22545118908596368No Hit
GACAAGGTCTCCAACTCTTGACCTCAAGTGATGCTCCCTCCTCACCTCCC52030.20575732973412897No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG50240.19867861322011604No Hit
GTCTAGAACTCCTGGCCTCAGGTGATCCACCCGCCTTGGCCTCCCAAGTG50030.19784814927154473No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC49710.19658268039753124No Hit
GTCCATAGATTCTGTGACAAAATTAACTACAGTCAGTCTGTGCAATGAAA48660.1924303606546745No Hit
GACCATGGTGGTGTGGCTGTCGGTGCCGACAAGGGTGTCGGGATAGGCGA47490.18780349008406275No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44440.17574198987862177No Hit
GATCACCCTATGTCCTCTGCTTGGAGAAATAATGAGAACGCCCTTTCCTT44270.1750697095393021No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC43130.1705614766756291No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42660.1687028192669218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.16435277001250045Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC40200.15897452729794317No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG39450.1560085846244741No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC39110.15466402394583476No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC39040.15438720262964434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG36610.14477754836760445No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG35710.14121841715944156No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33840.1338233334269253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.12733780544760623No Hit
GTACATGGGCACCGGTATGGACTACAAGGACCACGACGGTGACTACAAGG31000.12259229717005568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30420.12029863483590626No Hit
GCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGG29880.11816315611100851No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC29170.1153553970467911No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA28250.11171717403400236No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG27810.10997715433223383No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA27520.1088303231651591No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26740.10574574278475125No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC25910.1024634328927788No Hit
GAACAACGCCTCGGTCACCTTTGCCGGCACCACCCGCGGCAACGCGCTGC25870.1023052492835271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG25310.10009067875400353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)