FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364470
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT24729410.458749740956748No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA482412.040245805613943No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG275191.163854901944199No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC208890.8834537972568905No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA178270.7539533172338833No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA167230.7072620925619695No Hit
TACTAATACCATCCTACCAAAAGTAGAGTACTAACTTATACTTCCTTCTT164320.6949548947544271No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC143060.6050404530402163No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT139860.5915067647295166No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC129020.5456613955770214No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG127090.5374988898146308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116440.49245708340558353No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC112160.4743557752900227No Hit
ACATATACAACCTCCTGCCAAATCTCATGTGGCAAGTACTCTCATGATCC111240.4704648399006966No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT107990.45671968771014226No Hit
CGTGATGGCAGGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGA94740.40068175954865154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93580.3957757975360228No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT91280.3860484590627075No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG90170.38135396092993357No Hit
ACTCTGTTGACATCAGCCCCACACTTCAACAGGAGTGACACCAGGTCAGG78060.3301374092291296No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC77520.327853599326699No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC76440.3232859795218379No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG72180.30526925695821894No Hit
AGGCTCATGCCAGCACTGACACAAGTTCAAGCTGAGGAATGAGAAGCTGT65280.27608724153827285No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57990.24525580785546022No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG55350.234090514999133No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55000.2326102678401502No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC54570.23079167847339996No Hit
GCTGTTAATAGCACTAGTGGCTACACCCTTAGCGATAAAACCAAGGTCCT50890.21522793691609535No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG45670.19315110785926654No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT45520.1925167162197025No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT43960.18591904316823643No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42740.1807593244997822No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC42010.17767195185390383No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC41990.17758736630196195No Hit
GGTCTTTCCTGGATCCTTTGTTATCACCATCTCTCATGGAGGACTACGAA41430.17521897084758953No Hit
CTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAC41390.17504979974370577No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT40260.17027071605898997No Hit
CCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTT39190.16574538903009978No Hit
GTGACAATAATGGTGGAGCCGGTGTCCCTACCTAATGCTGTGATCCATTG39040.16511099739053572No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC38550.16303865136795984No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA35700.15098521021624298No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA34520.14599466265167246No Hit
ATGTATGATGGGTCAGCTACAGCCCTTGTCCATGCCTTCTTTGCTCCAGG34380.14540256378807936No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA34320.14514880713225375No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC33370.14113099341501478No Hit
TCTGTGGGTTGTGAAAACTGCGGGAAAAGCTCAGTATCTGGGCCGGAGTG33050.1397776245839448No Hit
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAG32620.13795903521719455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.13542146865893837Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA31680.13398351427592653No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA30890.13064238497422254No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC30230.12785106176014077No Hit
GTCTAAAACAATTAGGGCTCGTACGAGAAGAAGGGGGCTGGAACAAATAA30190.127681890656257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29930.12658227848101267No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA29480.12467910356232052No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29350.12412929747469834No Hit
ACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT29190.12345261305916336No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC28940.12239529365988995No Hit
CTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGA28750.12159173091644217No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC28550.12074587539702343No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG28160.11909645713415692No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA27560.11655889057590073No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA26960.11402132401764455No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC26700.1129217118424002No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACAACACGGGAAACCTCACCCGGCC26690.11287941906642926No Hit
GCACTTTGGGAGGCCGATGTGGGCAGATCACTTGAGTCCAGGAGTTCAAG26490.11203356354701055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26400.11165292856327211No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT26180.11072248749191149No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25580.10818492093365532No Hit
CACTCATTCCCTGGACCGTCAAACCTGAAATTGCTCCACCTCCAGAAACT24770.10475920608000948No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24710.10450544942418385No Hit
GCGTGGTGGCGGGCACCTGTAATCCCAGCTACTCGGGAGGCTGAAGCAGG24450.10340583724893951No Hit
GAAGTAATCTGCTTAACAATCTCAGAAGGACTGTGCAAGTCAATGAGTCG23890.10103744179456708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)