FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932959
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1557878.059508763507141No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA353601.8293197113854975No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT216441.1197340450573448No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG187240.9686703132347867No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC156300.8086048384885556No Hit
ATCATACTCACCTCGTCGCTATTAAAGCATGAAGACTTTCTGAAACCTGC155740.8057077258234655No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA117060.6056000153133098No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA112510.5820609749094523No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC100630.5206007990857541No Hit
TGCTCATATGGCGGCTCAGATGGCGGTACTAGGAAGTGGCTGTTCGGCGG96330.4983551125502404No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT85700.44336170606826114No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT85000.4397403152368985No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG84970.43958511277269724No Hit
CCTTAAATACTGTTCAGAAAGAATATATCTTCAATCAAGGCTCTTGTGCA84110.43513597546559446No Hit
CTTACGTAACTCACTGATGATCACACAGCAAGCAAGTGCTAGAGCTAGAA81200.4200813364380724No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC80560.4167703505351122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76940.3980425865214937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63290.3274254653099212No Hit
ATTAAGCCACTTTCCTTGCTCATTAGCATCTTGTAGAGCTGTGTGGCTAT60920.31516447063802183No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG59140.3059557910954138No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC58860.30450723476286873No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG56660.29312572072144316No Hit
CGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTG53780.27822628415812234No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA53070.2745531591720259No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG48600.25142799200603844No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC48530.2510658529229022No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA48290.2498242332092921No Hit
CTTGTATGTTAAAAGTGGGAGAAAAGGAGCTTTACTAATTTTTGATAGCT47210.24423694449804675No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG45230.23399358186076374No Hit
ACGCAGAGTACATGGGCAGCACGGGAGCCAGGCGCTGTGGACAGGGTCTG44030.22778548329271342No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT42050.21754212065543035No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC37440.19369267532317033No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA35820.1853117422563024No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC34650.1792588461524533No Hit
CTCTCTTAATTGAAAACATAATTCTGCCTCTTACTAAGGTCTTTGGGTGG33750.17460277222641557No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG32810.1697397616814428No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG31820.1646180803628013No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31340.16213484093558114No Hit
GTCAAGAATTTGAGATCAGCTTGGGCAACGTAGCAAGACCCAGTCTCTAC31190.16135882861457485No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC30620.15840998179475094No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC29120.15064985858468805No Hit
GGCGTGATGTCAGCTCACTGCAACCTCCGCCTCTGGATTCAAGCCATTCT28940.1497186437994805No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC26570.13745764912758107No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC26560.13740591497284732No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26210.135595219557166No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC25960.13430186568882216No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG25920.13409492906988715No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA25610.13249117027314083No Hit
AGTTAGCTCAGTCACGTTCCTGCCAGGTTGATGCTGGTTTTTGGCAGGAG25380.1313012847142645No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT25250.1306287407027257No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC25160.13016313331012194No Hit
TCTCACTACATTGCCCAGGCTGGCCTCAAACTCCTGGGCTCAAGTGATCC24760.12809376712077183No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24470.126593476633493No Hit
GTCTTAGAATGGAAAAAGTAAAGAAATATCAACTTCCAAGTTGGCAAGTA24060.12447237628940913No Hit
GTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTC23660.12240301010005904No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG23120.11960936574443638No Hit
CCTCAGAGTGGAGTTGAAACTGCTATAGCCTAAGCGGCTGTTTACTGCTT23080.11940242912550138No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT23010.11904029004236509No Hit
GTTGTCCGTGCTAGTAGCCTTCGCTTCTGGAGTACTCCTGGGCTGGCAGG22760.1177469361740213No Hit
CCGTCTCTACTAAAATACAAAAAAATTGGCCGGGCGTGGTGGTGGGCGCC22750.11769520201928754No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC22300.11536716505626866No Hit
CCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC22090.11428074780685984No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG22020.11391860872372359No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20950.10838305416721204No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA20590.10652062459679693No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20560.10636542213259567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.1049168658000506Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20150.1042443217885118No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC20060.10377871439590804No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19670.10176108236129168No Hit
CTTTTTTTATGAGCCCTCCACATCATGGCATCCTGTCAACATGGGAAGAA19590.10134720912342167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)