FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233913
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1519536.802100171313744No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA272811.2212203429587456No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG209360.9371895861656206No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC208800.9346827741277302No Hit
CTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTA196650.8802939058056424No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA147800.661619320000376No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT139230.6232561429205167No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC134400.6016348890937113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120580.5397703491586289No Hit
GCTTCTTCAAGGGGTCTACATGGCAACTGTGAGGAGGGGAGATTCAGTGT112160.5020786395889186No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT109950.49218568493938664No Hit
GGCTGGAGTGCAGTGGCATGATTTGGGCTCACTGCAACCTCCGCCTCCTG107940.4831880203033869No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA103990.4655060425361238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95670.4282619779731798No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC86060.3852432928229524No Hit
GAGCTCAAACTCTTCCAGGCACTTGGTGACATCACGAATCTTCATGGCAT83840.37530557367274375No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG81610.3653230900218585No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC81020.36268198448193817No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC77740.34799922826000834No Hit
GCTTTGGCCCCTCCTATCTTTCCTTATTTTCTCCATCAGGTTCTCTCCCT75530.33810627361047635No Hit
ACCATGCCCAGCTAATTTTCGTATTTTTAGTAGAGACGGGGTTTCACCAC71770.32127482135606894No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG64570.2890443808689058No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC61850.27686843668486644No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGTCCTT61070.27337680563209044No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT57980.25954457492301625No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC57250.2562767663736233No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55880.250144029780927No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC53630.24007201712868856No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC53480.2394005496185393No Hit
GCGTCAGATGTGTATAAGAGACAGCTCTATAAACTCCATTATTTCCAAGC50350.22538926090675868No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG50240.22489685139931592No Hit
GTGCTAGGGGGCTGGTTCTTGAGCATCTCACTCTACTGATGCTAACACCT48520.21719735728293804No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA46970.21025885967806265No Hit
GAGCCCCAGAGGTCAAGGCTGCGGTGATCCAAGATCATATCACTGCACTC46070.20623005461716726No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA41950.18778708033840172No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG40920.18317633676871034No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC38690.1731938531178251No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA37830.16934410605963618No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA37740.16894122555354663No Hit
GGGACTGAGTGTGGCAGGGACTCCCCAGCAGTGAGGGTCTCTCTCTTCCT34390.15394511782688045No Hit
CAGCAAAATTCTGTAAAGAAGTACACACATATTTCTCATTAAAGGCACCC34270.15340794381876108No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG33070.14803620373756723No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC32940.14745426522877123No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA32240.14432075018140814No Hit
ACTGGCGACAGGGAGGGCCCCAGCTGGCAGACCCCCCAACCACATCAGAG31450.14078435462795552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.13662125606503028Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30350.13586025955352782No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30310.13568120155082136No Hit
AGACAGGGTCTCGTTCTGTTGTCCACGATGGAGTGCAGCAATGCAATAAC29200.13071234197571704No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC28720.12856364594323952No Hit
CACTATAACCTCCACATCCCGGATTCAAGCGAGCGATTCTCCCACCTCAG27920.12498248588911029No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC27130.12144609033565765No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC26980.12077462282550844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26540.11880498479573734No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA26380.11808875278491149No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC24880.11137407768341918No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23760.10636045360763827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.10380887706907117No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC23090.10336123206230502No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC22750.1018392390393001No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT22750.1018392390393001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)