FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421599
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1403435.795468200969689No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA308541.2741168128992455No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC209760.8662045202364222No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG171120.706640529666555No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA149650.6179801032293125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142730.5894039434274626No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC136530.5638010256859207No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT135820.5608690786542281No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA134560.555665905048689No Hit
CCACCAGCCTGCGCAAGCGGGAAGGCGAGTATTCCAAGGTGCTGGCCATT121210.5005370418471431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119040.49157602063760353No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT115790.4781551363376017No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC95430.3940784580766675No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG82560.3409317562486605No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC80890.33403548646989034No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG70070.2893542655080383No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC65440.2702346672591127No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG64940.26816991582834315No Hit
GGCACTATCCTGGCTCTCTGCAATCTCCGCCTCCCGGATTCAAGCAATTC63870.26375134776649645No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA62630.25863076421818804No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA61040.252064854668341No Hit
GCTTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTG60310.24905031757941754No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC58060.2397589361409548No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC55900.23083920996003052No Hit
CTTGTCAGCTGTCTTTCAGAAGACCTGGTGGGGCAAGTCCGTGGGCATCA55710.23005460441633813No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC55400.22877445852926104No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55280.22827891818587637No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG51420.21233903714033578No Hit
CTCTAAGAAACACCATCACCTCCATGTGCCTTACAGAGGCCCCCTGCGTT50110.2069293883917197No Hit
GAACTCCTGATCTCAAATAATCCGCCCACTTTGGCCTCCCGAAGTGCTGG49090.2027172954729499No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44400.18334992705233197No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC41940.17319135001294597No Hit
GTACATGGGAATGGAATGGAATGGAATGCAATGGAATGGGATCTTCCGGA40640.16782299629294528No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC40560.16749263606402218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.15811866456832863Clontech SMART CDS Primer II A (100% over 21bp)
GGACTGAGGGGAGGGGAGGGGGAGGTTGGGAGGGAGCCGCCGCCGCGCGC37780.15601261810894373No Hit
CAGCAGATACCTTTACACCCTGGTCATCACTGACAAAGAGAAGGCAGAGA37190.1535762114206357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37190.1535762114206357No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA35140.14511073055448073No Hit
GGGCAGAGGAACCCAGAGCCCGGAGGTGGGATGCCCCTGAGCCAGCAGCT34700.14329374929540356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33160.1369343148886335No Hit
GTCTCGATCTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAAAGTGC32840.13561287397294103No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGCGGGC32470.13408495791417158No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA32450.1340023678569408No Hit
GGTTTGTAAAAAAACCATTTTCTTTAGCAAGTAGCTTGGGTATTTTTTTA32330.13350682751355614No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC31850.1315246661400174No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA29340.12115961395755448No Hit
ACTCCAGATCCAGATGCAAATGGCTTGGAATCTTACACAACTCTCCCTGA28850.11913615755540037No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC28620.1181863718972464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28360.11711270115324626No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATG27780.11471758949355364No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC27030.11162046234739938No Hit
GGGGTCCCACTCAGTTGGGTCACTTGCTGGACTGCTCCACTGGCCAGGGG26850.11087715183232237No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26820.11075326674647619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG26740.11042290651755307No Hit
TCAACGCAGAGTACATGGGTGAGCGTATAATCCATCCTAATCGTCAACGC25920.10703671417109108No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA25910.1069954191424757No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA25710.10616951857016789No Hit
AAACAAGTCTCAATTAATTTTGAGACTATACCAAGCGTGTTCTCTGGCAC25650.10592174839847555No Hit
ACGCAGAGTACATGGGCGTGGTGGTGCATGCCTGTAGTCCCAGCTACTCA25380.10480678262586002No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC25000.1032375715384752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)