FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130825
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG842693.9547593068412468No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC210270.9868008869803948No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA174930.8209496321847173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143960.6756068658852792No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA143890.6752783546279024No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT143310.6725564042096371No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC135460.6357162132038061No Hit
CTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGT123600.5800570201682448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115060.5399786467682706No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT109170.5123367709689909No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG98610.46277850128471365No Hit
GAACTAGGAAGTGATGGGGCCAGGTGTCAAACCCAGAAGGGCTGTACTTG92020.4318515129116657No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC82840.3887696080156747No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC81460.38229324322738845No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGCGGGCGCCGGCGGCTTTGGT80760.3790081306536201No Hit
TCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAGGTAAAGG80540.3779756667018643No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA75900.3562000633557425No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG59960.28139335703307405No Hit
TCCAATATCAATATCAATTCTTCTTTTCTTTTTTTGAGATGGAGTCTTAC59540.279422289488813No Hit
TCTTAATGCCTCGCGGTTAGCGTTGACGATTAGGATGGATTATACGCTCA58400.2740722490115331No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57300.2689099292527542No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA55360.2598054744054533No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC53600.2515457627914071No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC53230.24980934614527237No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG53190.2496216254267713No Hit
ATAGGAAACAAGCACAAAAGGCCATTGGGTGGGGCTGGATGGAGCATGAT50150.23535485082069155No Hit
ACATTGGCTACTTTGACAACCTTAAAGCGGACTCCAGGAATATCACCAAC49000.22995788016378632No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG48610.2281276031584011No Hit
TGGTGGCCCGTTGGTCCCATGAAATGGCTGGGTTGCAAGGGAGGAAGACA48530.22775216172139898No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA48260.2264850468715169No Hit
GCACCAACACTTGGTATTGCAGTTGTCCCTTGGTATCCACAAGGGATTGG48200.22620346579376532No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC45830.2150810132225781No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT44370.20822920699728975No Hit
CTTTGGGAGGCCGAGATGGGTGGATCACCTGAGGTCAGGAGTTTGAGACC42910.20137740077200145No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG41080.1927891779005784No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36290.17030962186007767No Hit
GTACATGGGAGGCTCTGGCCTGGGCACTCACCCCCTGGCTTAGACACCTT35920.16857320521394295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35830.16815083359731559No Hit
GGTCGATTTGGTGAACGGCTTTTCTGTCACAATCTTCAAGTTTCTTTATG35110.1647718606642967No Hit
CCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTC34590.16233149132378305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.15979726162401886Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33180.15571433599662102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30550.14337169875517697No Hit
GCCACAGCCCTTTCAAATCGACGATTTACCTTGCGCGGTCTGGATTGGTG30140.14144756139054124No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA29180.13694226414651603No Hit
AGTCTACTCTTACAGCAAAGCCTGATTATAAAGATAATAAGAAGACTTCT28840.1353466380392571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.13018431828047822No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26990.12666455480858352No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA26810.12581981157532882No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24650.1156828927762721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24330.11418112702826369No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC23320.10944117888611218No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC23020.10803327349735432No Hit
GAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACAGGGCCAAAAA22710.10657843792897118No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC22700.10653150774934592No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22560.10587448523459222No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21580.10127532763131653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)