FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172214
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1370766.310427978090556No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA341601.5725890727156715No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC197610.909716998417283No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG194910.8972872838495655No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA138540.6377824652635514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136620.6289435571265078No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC127460.5867745995560291No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT125070.575772000364605No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA123600.569004711322181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116990.5385749286212131No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT110760.5098945131557019No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC84560.38928024586896137No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG80690.37146432165523285No Hit
CATCAGTTCACCAACAAACACGATTCAGTGCTGCTAGTGCTGGGTCCTGT80290.36962288246001546No Hit
GTTCTGGAGATGGGAGTCCAAGATCAAGGTGCTGGCAGGGTCGATCGCAT77030.35461515301899355No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC73940.340390035235939No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC72560.33403707001243893No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG69590.32036438398794964No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA60870.28022100953220996No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57110.26291148109716633No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC56580.2604715741635032No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT53000.24399069336630738No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG47040.2165532493575679No Hit
CCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT42980.19786264152611113No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42530.19579102243149155No Hit
TAAGTAACTTGCCCAAGGTCACACCTCGAGCAGTGAATTCAGGATCCAAC41200.18966823710739367No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC40310.18557103489803492No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39280.18082932897035006No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA38880.17898788977513266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37940.17466050766637173Clontech SMART CDS Primer II A (100% over 21bp)
CCCGAGTAGCTGGGATTACAGGCATGAGCCACCGCACCTGGCCATTTCTT37570.1729571764107956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37230.17139195309486083No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG35020.1612180015412846No Hit
GATTTCACCTTCGAGGGCAAGCTGGAGGCCGCCGCCCAGGTGAGGCAGGC34480.1587320586277411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33380.1536681008408932No Hit
GTAGAGATGGGGTTTCACTGTGTTGGCAAGGCTGATCTCGAACTCCTGAC33110.15242512938412145No Hit
GTACATGGGAGCTCAGTGAATTTTTGCCAACTCAGCACGCACGTGTAACC31280.1440005450660018No Hit
CACTTGCGCTTACGGCAGTTTGTCGCACGCGGCGGCAAACGGGCGTAGCA31240.14381640114648006No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT29900.1376475798425017No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC29520.13589821260704518No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC28830.13272172999529513No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28750.13235344215625164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG27540.12678308859071896No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27420.12623065683215373No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC25660.11812832437319712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.11697742487618625No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC25390.11688535291642535No Hit
GTATCAACGCAGAGTACATGGGAGAGCTCTTCATGGTCTGCTTTTTCTGT24950.1148597698016862No Hit
CCACACAGAGAGGCTCAGGTGGAACCGGGTTCTGACCCAGAACCTTCTTG24640.1134326544253927No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24370.11218968296862096No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC23660.10892112839711005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23260.10707968920189263No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG23260.10707968920189263No Hit
CCTTCGCACTCTCTCCTGCTCCTCCATGATAAGATGTGCTTGCTTCCCCC23140.1065272574433274No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC22510.10362699071085998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)