FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703428
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1781376.589300695265418No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA412031.5241019919894299No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG232770.861017937226366No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC196650.7274097923081362No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC195980.7249314573940937No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA183950.6804323991613611No Hit
GTCGAGGCGCTCCCTCGGCGAGCCGGCGCCGCCGGCCGGGTCTCCTACTG176630.6533556654736136No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT166360.6153668601494103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147620.546047462702909No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC145250.5372808153204006No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA141920.5249631208968761No Hit
GTGAAACCCTGTCACTACTAAAAATACAAAAAATTAGCCAGGCATGGTGG123480.4567534256506924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.4342264709842466No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG110120.40733468766321873No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT106290.39316748957249836No Hit
CTCCTGCCTCAGCCTCCTGAGTAGCTGAGACTACAGGCGTGCACCACCAC93380.3454133048855009No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC92950.34382273173171246No Hit
CAGGAGGCTGAGGAGAATCATTGAACGGAGATGCAGGTTGCAGTGAGCTG87270.3228123700723674No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA85140.314933484450113No Hit
GGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAA81910.3029856907600276No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC81610.3018759885597101No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC77660.28726490958886275No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA72640.26869589277021616No Hit
GTACATGGGGACCTGTGTTCCCTCCCCGATCATCTTTCCTGTTCCAGAGA67350.24912814397128388No Hit
CAGAGTACATGGGCACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCT63570.23514589624728308No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG58170.21517125664156767No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA55930.20688548021253017No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG55600.2056648077921809No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC55170.20407423463839244No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA53280.19708311077639204No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA53050.19623233908948193No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT50650.18735472148694177No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG49610.18350775385917437No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC48520.17947583586468735No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG47510.17573983845695168No Hit
ACACTGAATCTCCCCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGCT47460.17555488809023212No Hit
GCGGAAATGGGTCGGTTAAGGCACCTGAAAATGCAGGGCGACGCCCCACC42510.15724480178499298No Hit
CCCGAATGCGGCACGCGCTCCGATATTTTCGGGCATGGCGGAGCCCGGCA42360.1566899506848342No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42020.15543228819114102No Hit
ATTAAAAACAGAGGGATAGAAGCACTATGAAAGCAAACTCATTCCCCTTC41040.1518072610034371No Hit
ACGCAGAGTACATGGGCCGTTGGGATCTAATCGTCAACGCTAACCGCGAG40900.15128939997662227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG38540.14255974266745777No Hit
GGCTGGCCTTGAACTCCCGACCTCAGGTGATCCGCCTGCCTCAGCCTCCC37220.13767705298606067No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA36730.13586453939220872No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC36560.13523570814536212No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35300.1305749589040285No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35230.1303160283906211No Hit
CACCAAGGATGAGCAGCTGGAAAGCCTATTCCAGAGGACTGCCTGGGTCT35030.12957622692374274No Hit
GTCCAAGGCCGGGTTACATTCGCTCGGCGGGGGCTTGTACCTCAGCACTT34610.12802264384329823No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.1279856537699543Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC33620.12436062658225039No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32750.12114249020132957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31490.11648174095999597No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC30910.11433631670604877No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC29800.11023041856487394No Hit
CTCAAGGGATCCTCATGCCTGTAATCCCAGCACTTTGTGAGGCCAGGGTA28990.10723422262401662No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28980.1071972325506727No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC28760.10638345093710651No Hit
CTCGCACCTCCAGGCGGCACTCACACCGTGCCTCGCCCTGTAAGTTGGTG28720.10623549064373083No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC28400.10505180829672549No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA27890.1031653145561857No Hit
GCTGAGGAGGGAGGATTGCTTGAGCCCAGGAGTTCCAGGCAGGAACAGGT27850.10301735426281003No Hit
CTTCTTGCCAAAGGTGGGCACAACGTTGACAAAGCGCCGGTTGTACTGCA27840.1029803641894661No Hit
CTCCTGCCTCAGCCTCCCGAGTAGCTGGGACCACAGGCACCCGCCACCAC27160.10046503920207973No Hit
GGGTATGGAGAGGATGGGGGTGCCCTACAGCTGAGTGCTGAGGCTGGAGG27050.10005814839529664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)