FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429162
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1170404.818122463631491No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA296251.2195563737618158No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC204640.8424304348577821No Hit
CGTCCTCTCCATTCCGGAAGCCGAGCGGCGCAACAAGGACATGCGCGCCG169050.695919004166869No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA152590.628159011214567No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT145900.6006186495589838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137920.5677678145796781No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA137620.5665328207834636No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC136720.5628278393948201No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG135820.5591228580061767No Hit
GGTAAGGGAAGAAGAGATAGGGAAGAGAGACAGAAAAGGTAGAATGCGGC122350.5036716365561457No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT111810.46028218784914304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.45929419281217143No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA105790.4354999790051055No Hit
GTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATG101860.4193215602746956No Hit
CCACCAGGAGTGGAGCCTGCGGCTTAATTTGACACAACACGGGAAACCTC89850.3698806419662419No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC78000.3210983870157692No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC73170.30121498689671583No Hit
TCATAACTCACTGCGGCCTCCAACTCCTGGGCTCCAGCGATCCTCTTGCC72510.29849800054504394No Hit
ATCCACATCCTCTACAGGTCGGGGACCAAAGGCTGATTCTTGGAGATTTA71230.2932286936811954No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG71000.2922818651040976No Hit
CCTGTAATCCCAGCTACTCGGGAGGCTGAAGCAGGAGAATTGCTTGAACT70940.2920348663448547No Hit
GTACATGGGAAGCACTAAGTGTCAGCTCTCTTCCTCCCTGACTGCAAGGC65000.2675819891798077No Hit
CAAGGGGGTTGCCTGCTTTGACTGAACTAACCTGTGGGTTTAGTCCAACG64820.266840992902079No Hit
CTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGA62580.2576197058903441No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA60120.2474927567613852No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59260.243952441212237No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG58300.24000046106435058No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC56820.23390782500302573No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT56100.23094383989211092No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC55030.22653902868561257No Hit
CAAACATCCATCTTTCCACATTGTTGAGGCCTAGAATGTTTCACTGTTGT54220.2232045454358334No Hit
AGTCAGGGGCGCGTCCTCGCAATACACATCTTGTTAGGGAAAGTGTTCGG52460.2159592484980417No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC49260.2027859813384204No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG48920.20138632170271067No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC44930.18496090421305783No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG41820.1721581351923009No Hit
GGTTTGGGGGGGATATGAGACAGCCACAGCGCGGGAGGCGCTGGACAGAC40960.16861781964315267No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCC39570.16289568172069216No Hit
CAGGGACACAAACACTGCGGAAGGCCGCAGGGTCCTCTGCCTAGGAAAAC36700.1510809077369068No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36640.1508339089776639No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG35930.14791109032662292No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT35830.14749942572788477No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35340.14548226919406773No Hit
CGGCGAGGATGTCGAGGTTCCCTATGTCCGATACACCATCCGCTGACCCC34670.14272411638252205No Hit
GATCACAGTGGCCACGGCCACGGCCACAGTCATGGTGGCCACGGCCACAG34220.14087162568820027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33600.13831930517602367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.13177383805608683Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA31620.13016834612100797No Hit
CAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA31610.13012717966113416No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30690.12633986535274305No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29850.12288188272334245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29150.12000023053217529No Hit
CTCTCTCTGACTCGGCATCGTGACTCAGTTTCCTCTTGGAGGACAATCGC29060.11962973239331096No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC28810.11860057089646554No Hit
GAGTACATGGGAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTC28020.11534842056643402No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG27820.1145250913689577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.10925578450510917No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC26100.10744446027066124No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC25460.10480980683873699No Hit
TTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCG25240.10390414472151302No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC24700.10168115588832692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)