FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004047577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054266
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1542175.049232778022608No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA317951.0410029774747844No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC237340.7770770456797148No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG176440.577683803571791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175240.5737548726928171No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA165140.5406863711281205No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT158140.5177676076674396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151000.49439046893754507No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC141970.4648252640732667No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT130360.42681285781919454No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA121240.39695298313899313No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA116700.38208852798020865No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA101750.33314059744632585No Hit
TCGCGGGAATGAGTATCAGCCGAGCAACATCAAACGCAAGAACAAGCACG95770.3135614252327728No Hit
GGTTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCATGATC88150.28861271415128875No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC87160.2853713461761353No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC86770.28409444364046876No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC85400.2796089142203069No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG82450.2699502924761628No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG82450.2699502924761628No Hit
GGGGCTTTCCCCATGTTGGCCGGGCTGGTCTTGAGCTCCTGTCCTCAAGT75260.24640944829297778No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC67150.21985642376924605No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA66850.2188741910495026No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT63580.20816785440429877No Hit
CAATTATCTGAAAGGCCGCCTGCGCTCGCATCCGGATGTTGCAGAGCACC62100.2033221729868977No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT60870.19929501883594944No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG58250.19071685308352318No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC57840.18937446836654043No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57340.18773741383363465No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC56500.18498716221835296No Hit
ATTGAGAATGGTTTCCTAAGACTGGTCATCCTGCTTCTACTCTCACTTCC54290.1777513811829094No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA52420.17162879722984178No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG48470.15869606641988615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.15758286933751023Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC48010.15718997624961284No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG47110.15424327809038244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG46220.15132932102181015No Hit
CCCCATCCCACTTGGAAAACTCCAAGGGGGTGTGGCTTCCCTGCTCACAC44960.14720394359888758No Hit
GAACCCAGGAAGTAGAGGAGTTGCAGTGAGCCCAAGATCACACCACTGCA42160.13803643821461523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG41070.13446765933288063No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA36990.12110929434436948No Hit
CCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCTG36620.1198978739900192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.11711488128407939No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG34670.11351336131168667No Hit
GGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT33850.11082859187772119No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG33620.11007554679258454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG33210.1087331620756018No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA32930.10781641153717456No Hit
GCAATCGCAGGCACTCGGCAGGCTGAGGCAGGAGAATCAGGCAGGGAGGT31640.10359281084227766No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC30750.10067885377370536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)