FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004047578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917231
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1331794.5652538314586675No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA335401.1497204026695178No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC274210.9399667012999655No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT237640.8146080992557668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200120.6859929844431244No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACA187420.6424585505912971No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA186050.6377623163883833No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC170570.5846982978036365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170520.5845269023947709No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT152510.5227902761214316No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG150950.5174427393648292No Hit
GTACGCAGACGCCAACGGCCCGCAGCGGCCCTGGTCGCTCGACCTGTTCC131440.450564250825526No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA126750.4344873614739456No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC123220.422386845608044No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC110580.37905808624685533No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA98600.3379917462826907No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG87990.3016216405214397No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG79500.27251870009608425No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC76970.2638460924074919No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC74900.25675032248046176No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT73230.25102571582435534No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG71920.24653515611208027No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG67430.2311438483959618No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC64980.22274547336155415No Hit
GGGTTCCTCTATATGGAACACGGGACCTCCCCACCCCAAGCCTAGTGCCT63530.21777500650445578No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA62250.21338728403749993No Hit
CTTTAAGAGGCAGGCCCAAGAAGGGAACCCCAGGAAGCCCTAGAAGCTCC61710.21153621362175296No Hit
GTCCTCTGCCTAGGAAAACCAGAGACCTTTGTTCACTTGTTTATCTGCTG58230.1996070931647168No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG57880.19840732530265856No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC55090.1888434614879658No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.18596401861902606Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG51780.17749708542107223No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC51770.17746280633929915No Hit
GGCTAGAGCAGAGACTGGGGCTGGGGCTGCTATGAAGATGCAAATGGCAG51600.17688006194915656No Hit
CTCTTACTCCCCACGGGCAAGGCTGGGTTTTGGTGAGGGAAAGAAAAGAC50140.1718753160102851No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC48900.16762470987042166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG47030.16121452157885338No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA46940.16090600984289555No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC44230.15161637868238753No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC42790.14668019090706222No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC42280.1449319577366345No Hit
CCGTCTCCTCTCTCCCATCCTCCTGCCATCCCCGATCTCTACTTTGCATT39900.1367735362746385No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG39660.1359508383120843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.13447683779584133No Hit
AAAAAAGACTCAAAATGAGCTAGGCCTGCCTAGTTAAAAAGATGCAACCT37990.13022623165597788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG36940.1266269280698032No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA36040.12354181071022485No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC35500.12169074029447788No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA35230.12076520508660438No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC33880.11613752904723691No Hit
GTTTTGTTCCACAGCCTCCACGAAGAGCTCATAGTCCCCACAGTACTGGT33320.11421790046794375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG31460.10784199125814856No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30930.10602519992417468No Hit
CTCCAGGCTCGCAGAGACGCCCGGCCGGACCCTCCCGCAGCCCGCAGCGC30880.10585380451530921No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT30680.10516822287984737No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG30330.10396845501778912No Hit
ACGCAGAGTACATGGGAGATGTGGTTTGACTATTTCTGTATGTCTCCATC29750.10198026827494977No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA29610.10150036113012648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)