FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708060
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1619975.982031417324579No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA347001.2813600880335No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG229950.8491318508452546No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC214610.7924861339852145No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC192730.7116902875120935No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA159670.5896102745138586No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA151750.5603642459915955No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT149470.5519449347503379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146650.541531576109835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127290.47004128416652513No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT127150.4695243089148689No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT105600.38994704696350896No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA92750.3424961042222107No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG85790.31679504885416127No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC84730.3128808076630503No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC83560.30856037163135236No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC76720.2833024379075796No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG76360.28197307297474944No Hit
GCCGCAGGGTCCTCTGCCTAGGAAAACCAGAGACCTTTGTTCACTTGTTT74750.27602785758070353No Hit
GAGGTTGCTGCTGCTGGTAAGGAGACTGAGCCTGTGGCTGTGAGTAGGGG70430.2600754783867418No Hit
TTCCTGGACAGCTAGAAAACACAAAATCTTGTAGGTCATTGCACCTATCT68070.2513607527159664No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA61850.2283922808209567No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC61310.22639823342171148No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG59410.2193821407206635No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT58200.21491399747420661No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC58140.21469243665206827No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG53400.197189131703138No Hit
GTCATGAAAGTTGGTAACACCCGAAGCCGGTGGCCTAACCGTTGTGGGGG50700.18721889470691197No Hit
CCCCAATTGTCCTGTGGGAATCTGGGCATGAAGGCTCTCCTGCCTGCTCC47840.17665782885165027No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA46420.17141422272770912No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC46020.1699371505801201No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG45440.16779539596611595No Hit
GAATACGGTTAGTCTGGGATACATATATTTTAGAGCAAAAAACAAATGTC44020.16255178984217483No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAA42100.1554618435337474No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC41450.1530616012939152No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC41420.152950820882846No Hit
GGCTGGAGTGCAGTGGTGCGATCTCGGCTCACTGCAACCTCCGCCTCCCA41280.15243384563118986No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC41000.15139989512787752No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.14778106836628435Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG39080.14430994881945008No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA38060.140543414843098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37810.13962024475085485No Hit
GACACAAACTGATAGACAACACACTAAAAGTTTTCTAATCCAAAGGAGGC37480.1384016602290939No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG36500.13478283346750072No Hit
GCCACAGAACAGGAAGCGGTCATGAAAGTGCCATGGATGAACACTGGAGG35800.13219795720921987No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG35680.13175483556494316No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA34320.1267327902631404No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC33600.1240740603974801No Hit
TGGCAAAACCCTGTCCCAGCCTAAAAACTACAAAAATTAACTGGGCAAAG32140.11868274705878008No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA32020.11823962541450336No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT31040.11462079865291021No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC30620.11306987289794171No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC29860.1102634358175225No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAG29840.11018958221014306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.10878636366993347No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC29380.10849094924041565No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC29330.10830631522196703No Hit
CTGCCAGCGTTAGCAACAAGCCGTATCCAGGAACCAAATCGAGGCCGGTC28640.10575836576737592No Hit
ATATCACGAGTGTGTGTGGCAACACTGCCGCATGTTAATCCTGCCTGCCT28040.10354275754599235No Hit
GTACATGGGAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC27630.10202875859471355No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27440.10132714932460876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG27370.10106866169878068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)