FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855449
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2571529.005659004941078No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA497241.7413723726111026No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG305881.0712150698541631No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC267210.9357897829728354No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA190830.6683012023678238No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT180020.6304437585822755No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC169960.5952128719511363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168380.5896795915458479No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA168030.5884538648737905No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT143530.5026529978297634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142830.5002015444856484No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG130570.457266090201576No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC117460.4113538711425068No Hit
GTGACGTGCACCTGTAGTTCCAGCTACTTGGGAGGCTGAGGCAGAAGAAT115870.4057855699751598No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC102620.3593830602472676No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC100680.352589032407863No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG99680.3490869562019844No Hit
GTGGCTCACGCCTTTAATCCTAGCACTTTGTGAGGCTGAGGCGGGCGGAT93600.3277943328702421No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT89240.3125252806126112No Hit
ACGCAGAGTACATGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA84220.29494485805910037No Hit
CCCATGTTCAGGTGATTCTCCTGCCTCCCAAGTAGCTGGGATTACAGGCA80690.2825825290523487No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC79620.2788353075120585No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC76020.2662278331708954No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT73790.258418203231786No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG71500.25039844872032385No Hit
TGTTGAATGCCTTTGTGACTTTAAAAATAGAAATAGGGGCCAGGCCTGGT70770.24784193309003244No Hit
GCTTGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTT68360.23940192943386487No Hit
TCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG67380.23596989475210378No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC66460.23274798464269542No Hit
ACGGAAAACTTAGCCAGGCATGGTGGTGTGTGCCTGTGATCTCAGCTACT66200.23183744482916696No Hit
GGCTGGAGTGCAATGGCGCCATCTCGGCTCACTGCAACCTCCGCCTCCGG63550.22255694288358854No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG62060.21733884933682934No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC59100.20697270376742852No Hit
TTCTTTCCCCTTTTGTCTTGCCCACTCCTGTTTACCCTTCAAGTTTCAAG58670.2054668109989007No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG56570.19811245096655553No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA53690.188026471493625No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG51210.17934132250304594No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA48660.17041102817805534No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA47250.16547310072776647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.16106048470835935Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG44920.1573132631680692No Hit
CTGCTGAAGGGCCAGACACATTCCAGCAAGGAGGGGTGTGGGGTCTTTCA44820.15696305554748133No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC44350.15531707973071837No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA42480.1487681972257253No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC42020.1471572421710211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG41570.1455813078783757No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG41260.14449566425455332No Hit
TTTTAGTAGAGACAGGTCTTCACCACGTTGGCCAGGCTGGTCCCGAACTC40490.14179906557602676No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG40460.14169400328985038No Hit
AAATCTAATGTAACCAAATTCCCAAATCCCATTCTGAGGCTCTCCATGTC40340.14127375414514495No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA38880.13616072288456213No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA38530.13493499621250457No Hit
GTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTC37390.13094262933780293No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAATGGTGGATCCG36420.1275456154181006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.1236933315916341No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA35080.12285283330222321No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA33660.11787988508987553No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC33300.11661913765575922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG33190.11623390927311256No Hit
TTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG33060.11577863936634834No Hit
GCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG32870.11511324488723139No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32810.11490312031487869No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32130.11252170849488119No Hit
TACTTAGCCTCCAGATGCATTTCCCAGAAATGGCATATGCCATTCAAAGG30980.10849432085812073No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCATAGTGG30780.10779390561694502No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA30560.10702344885165169No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG30180.10569265989341782No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29900.10471207855577179No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29590.1036264349319494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29460.10317116502518517No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG29200.10226062521165673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)