FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004047583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988251
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2399918.031152670910174No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA523781.7527978740741659No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG281950.9435285054702567No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC256600.8586962741750944No Hit
CCTTTGTGGGATACCCTTGGCTTCCTCCCCTAGCCCTCCTCCTCCTTCCT194910.6522544458280111No Hit
ATTTCTTGCCTTCTGCTAGCTTTTGAATTTGTCTGCTCTTGCTTCTCTAG190410.6371954698584557No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA187870.6286955145334178No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA174970.5855264500873588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173630.5810422216875356No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT164540.5506230902290337No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC161140.5392451972742583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148440.4967454206490686No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT135140.45223778056127145No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG128610.43038553320989437No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC113800.38082477007453525No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC107750.36057881349324405No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC99560.33317147722865315No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG92200.3085416854206692No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC86590.28976816204529005No Hit
GATAAGGTACATGCAGGTTATGAGTCAGCTCTCTGTCGCTCCTTCTTGTA83140.27822294713529755No Hit
CGCTTCACCGGCGTGGCCGGAACGGCAGCACAGGCTCCCAGCCCCGCGCT75000.25098293282592393No Hit
CACCAGGCCTGGTAGAATACTGGGCAGGCGTAGCCGTTGGAAATACCACA72470.24251644189192942No Hit
CAGCCAAAATTCGAGACCCAGCTCTGTTACCATCTAGCATTGTGACCCTG71030.23769756958167165No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT69760.2334475919191527No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC69150.23140626406550185No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC66710.22324095265089847No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG62260.20834929863656032No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61390.2054378966157796No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA60340.20192413555621666No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG52630.17612309006171167No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC51660.1728770441304964No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA50620.16939674746197692No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49600.16598337957554438Clontech SMART CDS Primer II A (100% over 21bp)
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG48310.16166647313093846No Hit
ACATTGAACGGGTGCAGCGGGAAAAGGCTAATGGCACAACTGTCCACGTA48010.16066254139963476No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA46830.1567137432565069No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC45980.1538692700178131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG45920.15366848367155236No Hit
GGGTCTGTCTCAGTCACGGTTCTCCCCAGCGATGACCGAGAACTGGTCCC41740.13968036821538754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG41660.13941265308703987No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC41610.13924533113182258No Hit
TGCGCACTGTGCCCACCGTGACTGGAGAATTCGACTCGGCAAGGTCGGTG41610.13924533113182258No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA41600.13921186674077912No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA41230.13797368427217124No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG40570.1357650344633031No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG38450.12867058356209035No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT35410.11849740868487954No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA35360.11833008672966228No Hit
GTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACAT35100.1174600125625324No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCCTGTCTCTTATA34960.11699151108792401No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC34500.115452149099925No Hit
ACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGC34170.11434782419549093No Hit
GGTGGAGGTTGCAGCGAGTCAGGATCGCAGCACTACACTCTAGCCTGGGT33920.11351121441940454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33730.11287539098957886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG33470.11200531682244899No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC32410.10845809137184259No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32050.10725337329427817No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA31880.1066844786465394No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC31210.10444236444662781No Hit
CTTTTTCCTTAGGGGCATCATGGCGTACAGGGCCCCATGTACCTGTCTCT30670.10263528733028116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)