FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768983
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1699816.138752025563176No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA387941.4010197967990414No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC246680.8908685968819599No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG202110.7299069730655624No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA178920.6461578131754511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175940.6353957391576619No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT169020.6104046142572923No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC152650.5512854358441348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146890.530483574655388No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA143320.5175907544394458No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT141030.5093205700432253No Hit
GCTGACACCCGGCGGCTGATCACCAAGCCGCAGAACCTGAATGACGCCTA122700.4431229805311192No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC95600.34525311278545223No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC94060.33969150406484977No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG86560.31260574730866897No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC85090.3072969389844575No Hit
GAAAAGAAATTTATTTGTCACAGTTCTGAAGACTGGGAAGTCCAAGATCA80160.28949256821006125No Hit
TTTTCAGATTCCACAAGGATTTATTGGCACCGGACCTAGCCTGGTGCCCG79800.2881924518857646No Hit
TTAAGTGTACTTATTGGCAGGGTGAGGCAGCTCACACTTGTAATCTCAGT77090.2784054651111979No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA76470.2761663758860203No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG73160.2642125285709591No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC69680.2516447374360911No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT64890.234345967454477No Hit
CAGTTGGTGTGATAGAATGACCTGCCTTTCTACTACAGAAGATTCTCATA62010.2239450368601035No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC60650.21903348630164937No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG57240.20671849556317248No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA56430.20379323383350492No Hit
CTCGCTCTGTTGCCTAGGCTGGAGTGCAGTGCTGCAATCTTGGCTCACTG53990.19498133430216075No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC52560.18981698334731562No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG51380.18555549095100982No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG49780.17977719617635787No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.1718320408612115Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC45920.16583706003251011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG45680.16497031581631233No Hit
ATCAACGCAGAGTACATGGGAATTCCGATAACGAACGAGACTCTGGCATG42500.15348595495169165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG41210.14882720478962855No Hit
GACTGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAA40470.14615474345635202No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC40450.14608251477166886No Hit
CATGTATCCCTGGTAGAAGGTGCCCCTGAGGAACATTTTGCAGGCTTTGC39880.14402399725819914No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG39320.14200159408707097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC38790.14008753394296752No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA38440.1388235319610124No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA37140.13412866745660773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.12900043084410415No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG34920.1261112834567782No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC33390.12058578907851727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG32650.11791332774524076No Hit
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCAC31760.11469915127684063No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA30650.11069045927692585No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACGAGGTCA30460.11000428677243594No Hit
CTTTAACATGGAATGATTTTTTGCTTTTTTTTGTTTTAATCTGAGATCGT29490.10650119556530323No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC28560.10314256172753679No Hit
GCCAAATACTGCATTTCGAACTTCGTTAAAAAAAGCAGCTCCAGCTCTTC28550.10310644738519521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG28520.1029981043581705No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC27880.10068678644830971No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA27870.10065067210596815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)