FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741413
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1918506.998215883560777No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA341881.247094108038446No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC273600.9980254708064783No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG215030.7843765240771822No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA194430.7092327934535949No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT189770.6922342602154437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176900.6452876673452704No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC176150.6425518519099457No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT149410.5450109122558331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148110.5402688321679368No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA139520.5089346260486837No Hit
GTCATGCCCTCCTCTCCCATTGTCCCTCTGCCCCATCCTCCAGCAGGGAA137050.4999246738816807No Hit
TCCTTTCACTCTGGTCGAACCATCTGTTTTAGCCCCTTTGTCTGTCTCAC120470.4394449139914343No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC105910.38633361700699603No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC105830.3860417966938947No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG103120.3761563835875879No Hit
GTGTAAGGAAGGGGTCCAGTTTCAGTGTTCTGCATATGGCTAGCCAGTTT98290.3585377321840963No Hit
TGCTCTCAGTACCCCCGCCTTCTGTTGTCTGCTGTTGCCTGCTCCCTCCC95370.3478862907558985No Hit
GTGCAATGGCATGGTCTCGGCTCAATGCAACCTCCGCCTCCCGGGTCCAA93280.34026248507612683No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG92160.3361770006927085No Hit
GAATGTATGTTATATCAGTGATTGGTATATGATAAGAGTTACATAAATGC89050.32483248602089504No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG83540.3047333619560424No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT74990.2735450659933399No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC74350.27121050348852943No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC73420.2678180923487267No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA68630.25034535110178585No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC63410.23130407567192537No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG59070.21547282368617934No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC55920.2039823988578153No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA51290.18709329823707702No Hit
GCACAACCACCACAACCGGCCTCAGGGTCACACAGGGCAAACGACGCTCA50250.18329963416675998No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.16495143198051515Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG44730.16316403256276965No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC43580.15896911556193832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40790.14879188214253014No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA40640.14824471905546519No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA38030.13872408134053496No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA37820.13795805301864403No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC36590.1334713157047114No Hit
TTCTGGGTGTGTACTTAGTCTCTCTGAGCCACAGTTTTCTGCTCTATAAA36570.13339836062643606No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC36440.13292415261764645No Hit
TGCTTCCGGATGACTGACCTCTCAAAGTGCTGGGACTGTCTCTTATACAC36170.13193925906092954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.12767138698182287No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG34870.12719717897303326No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC33050.12055826684997846No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT32190.11742119848413939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG31480.11483129320536527No Hit
GGCCTTACATATTTATTACTGAATCCAGCCAACCAACGTGTTCATAACAG31320.11424765257916265No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC31240.11395583226606135No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC30500.11125649436987421No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG30360.11074580882194693No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG29980.10935966233471571No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG29590.10793703830834682No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG29040.10593077365577531No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC28540.10410689669889214No Hit
CATTAGGGTTGAGGCCGTGTTTTGTTCACCCCTATGAATACAGCGCATGC27770.10129812618529205No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG27520.10038618770685044No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA27450.10013084493288679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)