FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004047590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574870
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1836085.136074878247321No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA337320.9435867597982585No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC261450.7313552660656192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205920.576020946216226No Hit
AGGCAAGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACTAATGTGG193860.5422854537367792No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG193820.5421735615560845No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA189460.5299773138603642No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT176660.4941718160380657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166210.4649399838315799No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC163370.4569956390022574No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT141360.3954269665750083No Hit
TACAAATCTGGCTGTAAGAGGCAGCTCCTTAGCTTACTGCCTCATTCGCA115540.3232005639365907No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA112200.31385756684858473No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC111370.31153580409917003No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC105510.29514359962739906No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG89210.24954753599431587No Hit
GCCCAAGAGTACGGAACTCGGCAGAGATGTACACCCGCCATCCCGTCTCC84800.23721142307272713No Hit
GTACATGGGAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA84750.23707155784685874No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG80540.2252949058287434No Hit
ATTCATTGGTTGAAAAAGCCCTGCCAAGTGAAGGACTCTTCTACCCAGGA79970.2237004422538442No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT79300.22182624822720826No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC74140.2073921569175942No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT71140.199000243365493No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG64770.1811814135898648No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA64290.1798387074215286No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC62050.1735727453026264No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC56110.15695675646946602No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG54250.15175377006716328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG52260.14618713407760284No Hit
GCCTTCCAAAGAAACGATTGAACAGGAGAAGCAAGCAGGCGAATCGTAAT52130.1458234844903451No Hit
GGGTCTCACTCTGTCACCCTGGCAGGAGTGCAGTGGCACAATCACAGCTC50860.14227090775328893No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA50110.14017292936526363No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.13807495097723832Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC49350.13804697793206466No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49300.13790711270619632No Hit
GGCTGAAGAGCAGCAGTGGTGCGATCTCAGCTCACTGCAACCTCCACCAC47200.13203277321972548No Hit
TACTGGGATTGGCTCCCGAGGGCAGCGAGGAGGGAGCTGAGGCACGCGGG44390.12417234752592403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG44200.12364085966762428No Hit
GAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGGTATTGCAGTA43880.12274572222206682No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC43590.12193450391203037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.11519300002517574No Hit
CATTGAGCCATCAAGAAGGAAAAACCACTCAGACCGCGTTCTCTCCCTCT41040.11480137739274435No Hit
CCATTCTAACAATGGACATGCTCACCGTGAAGGTGGGTGAGCCCAAAGGC39640.11088515106843046No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39620.11082920497808313No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG39240.10976622926148363No Hit
TACTGGGATTGGCTCCCGAGGGCTGTGAGGAGGGAGCTGAGGCACGCGGG38360.10730460128620062No Hit
GGTCCAGAAGGCCCTACAATCCTTAGTGACCTAAGGGCAGACAAAGGGGG37760.10562621857578038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG36700.10266107578737128No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA36580.10232539924528725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)