FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427928
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC27910311.495522107739603No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA569782.346774698425983No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG369011.5198556135107795No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA210170.8656352247677854No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC198780.8187227957336461No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG149280.6148452507652615No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA147750.6085435811935115No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT138680.5711866249740519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136150.5607662171201123No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC127570.5254274426589257No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC123490.5086229904675921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119410.4918185382762586No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT112400.46294618291810957No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC86200.35503523992474245No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG86050.354417429182414No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC79210.3262452593322372No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG72070.29683746799740357No Hit
GAGTTGATTTGGGTACCTTTAGAAAATAGTGGAGGAAGCTGGGTGCAGTG71140.2930070413949672No Hit
TGTAATCCCAGCACTCTGGGAGGCTGTGGCGGGTGGATCACCTGAGGTCA64760.26672949115459765No Hit
GGCTCTTCCAGTGCTAGGAGAGGTATGAGCAGCCTCTCACCTGTGAGCTG58400.24053431567987188No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC58350.2403283787657624No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57380.23633320263203852No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC57150.2353858928271349No Hit
GACCTGTACGCCAACACAGTGCTGTCTGGCGGCACCACCATGTACCCTGG56810.23398552181119045No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG56060.23089646809954822No Hit
CTGAATGCTGCGACCACTCCTGTGGTCCGACTCCTTGCCTGCGACCCTCA55340.22793097653637176No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC51630.2126504575094484No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC51270.21116771172786014No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC51060.21030277668860034No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG48810.20103561555367377No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA46590.1918920165672129No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA45930.18917364930096772No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC45730.18834990164452983No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT41930.1726986961722094No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG41820.17224563496116854No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC41390.170474577499827No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA40100.16516140511580243No Hit
TACTTGGGAGGCTGAGGCCGGAGAATGGGGTGAACCCAGGAGGCGGAGCT39630.16322559812317333No Hit
CTTCCCTCAGGAGCTTCTAGATGCCACCCAACACACACTGCAGCAGCAGA39520.16277253691213248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37750.15548237015265692No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA37590.1548233720275066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36580.1506634463624951Clontech SMART CDS Primer II A (100% over 21bp)
GTCTTGAACTCCAGACCTTGTGATCCACCTGCCTTGGCCTCTCAAAGTGC36560.1505810715968513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG36540.1504986968312075No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT35870.1477391421821405No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35090.1445265263220326No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG34200.14086084925088388No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA34120.14053135018830873No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA32560.13410611846809295No Hit
CCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTTGATACTGTCT32370.13332355819447694No Hit
GGACCGGAGGGCCTGAGAACCCCCAGAAGGACACTGCTGGTCCTGTCTCT32020.13188199979571058No Hit
AGCTAGGACTGAGGAGCAAGCGAGCAAGCAGCAGTTCGTGGTGAAGATAG29280.1205966569025111No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA29100.119855284011717No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28800.11861966252706012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28490.11734285365958133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG27380.1127710541663509No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCGCAGACCTGTTA27350.11264749201788521No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC27090.11157662006451591No Hit
AGTCAAACTTATTCGAGTTGTCCACAGTCAGCAATGGTGATCTTCTTCCC26480.10906418971238026No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG25770.10613988553202565No Hit
GTACATGGGAAAGTAGAGTTTGTTCAGAGGAGAGCAACTAAGTTACCTAG25710.10589276123509428No Hit
GGGCAGTTCTCACTTGGGATGTATCATGCGGTATCAGTCATATGTTAGTG25620.10552207478969722No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25170.10366864256271191No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA24970.102844894906274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24720.10181521033572659No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC24710.1017740229529047No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC24450.10070315099953542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)