FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004047594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3093656
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2332987.54117458437525No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA487071.5744155135541897No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG261960.8467651219140072No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC202600.6548885848976098No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA190420.6155176916890566No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT162470.5251715122819085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157080.5077487606896177No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC154610.49976467971875344No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA153160.49507766862249714No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC136000.4396093166143877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128310.41475199569700055No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC121300.3920927213626854No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT114790.37104965775121734No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG114000.36849604480911907No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA103850.33568696713532464No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC89310.2886875593149335No Hit
GTCCTCATCGTCCCCGAGGTTGACGCCGTCATCACTATGACCAACGAGGC84720.27385074487919797No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA84470.27304263951777447No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC76560.2474741858823347No Hit
GCTTTACAACCCGAAGGCCTTCATCACTCACGCGGCATCGCTGGTTCAGG72670.23490006645858494No Hit
GGCCGGGAGCATATCCGGGCTGCATGTGGCCTGCTCCAGCCAGACAGCTC72360.23389801581041977No Hit
ATTTATAAGTAATAGTACATCTAGCCGGGCGCAGTGGCTCACGCCTGTAA71610.23147369972614926No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG70010.2263018254130388No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG61960.20028083277520192No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT58390.1887410882140742No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC58380.18870876399961728No Hit
GTACATGGGGTGGTGAAATGAGGAGGAGCCAATTTAAGAAGACCCTTATG57110.18460358876358587No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC56360.18217927267931536No Hit
GTTCACAGGTGTTTCTGCGACAGGTGAATGACGGGCGTGGGTCGGTGCGC53710.17361335584822618No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG51470.16637273180987155No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC51040.16498279058822313No Hit
GTAAAGATAACTTGAGGCCAGGAGTTTGAGACCAATATAGGCATCAAAGC49320.1594230257016294No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT49200.15903513512814613No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG48030.1552532020366841No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA47860.1547036903909161No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT46440.1501136519380306No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC45630.14749539056701844No Hit
GGATTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGA45230.14620242198874084No Hit
CTCAACGTGACGGGGAAGTGGTTCGGGCGGCCGCGGCTTACTACCCCAGG44550.14400437540566888No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44530.143939726976755No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC44250.14303464897196067No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA43210.1396729306684389No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC42320.1367960755817712No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG42300.13673142715285733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42280.13666677872394345No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG41790.13508289221555336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.13049285376266787Clontech SMART CDS Primer II A (100% over 21bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACTCGTCTGTCTCTTA39620.12806853767839732No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA38490.1244159014447631No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT37770.1220885580038634No Hit
CTCCTGCCTCAGCCTCCCGAGTAGCTGAGGCAGGAGAATGGCGTGAACCC37690.12182996428820787No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT36330.11743387112206399No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT36070.11659344154618355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35360.11429842231974079No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC34640.11197107887884108No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA34390.11116297351741757No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC33100.1069931498524723No Hit
GTCTTGCTCTGTTGTCCAGGCTGGAATGTCCAGCTAATTTTGTGTTTTTT32790.10599109920430715No Hit
CCTCTGGATGCGCCCTGTGCGAGCAAAGCCGGGAGAGCGAGCCCCAAGCA32780.1059587749898502No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA31930.10321121676101028No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC31610.10217684189838819No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC31170.10075457646228281No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG31020.1002697132454287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)