FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512132
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2227958.868761673351559No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA443571.7657113559319335No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG268221.067698671885076No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC203880.8115815570200928No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA168420.6704265540186582No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA141540.563425807242613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137590.547702111194794No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT136510.543402974047542No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG128110.509965240680028No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC124930.49730667019089764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120070.47796055302826446No Hit
TTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAG117050.46593889174613434No Hit
GAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCACCTCCCAGGTTC113200.4506132639526904No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT109890.4374372047328723No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT88170.3509767798825858No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC85870.3418212100319569No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC83430.33210834462520283No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC76620.3049998965022539No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA74530.29668026998581287No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG68730.2735923112320531No Hit
GGCCCAGTTTTTGGGTGCAGATGATTCCAGAGTCATCCGCAACGCCCTCC64520.2568336377228585No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT59580.2371690659567252No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA59390.23641273627341236No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC58660.23350683801647365No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC58040.23103881483934763No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57290.22805330293153386No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG53310.21221018640740213No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG50940.20277596877871068No Hit
GGGACATGCATTGATACTTTAGTAATTTGAGGGTACATTTTGAGTGTTAA48940.1948146036912073No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG45190.1798870441521385No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC44280.17626462303732446No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC43610.17359756573301083No Hit
ATCTACTGGCATCCCCATGGAGGCCCTTTCTCTCCCCAACTTTCCCTCGT43060.17140819033394744No Hit
GTACATGGGAGGAGAATGGCATGAACCCAGGAGGCGGAGCTTGCAGTGAG43000.17116934938132233No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG41570.1654769733437574No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG41500.1651983255656948No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG41320.16448180270781948No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC40570.16149629080000572No Hit
GGCTAGAAAAGCCAAGGCCAAAGGCTGGAGGGGAGAGGACAGTCAGCATG40420.16089918841844297No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC38180.1519824595204392No Hit
ATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGCTGTCT38120.1517436185678141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37780.15039018650293853No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.14891733396175041Clontech SMART CDS Primer II A (100% over 21bp)
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC36490.14525510602149885No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA36140.1438618671311858No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC35960.1431453442733105No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA34870.13880640030062114No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG34050.13554224061474476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34000.13534320648755718No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC33820.1346266836296819No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA32950.1311634898166179No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG32460.1292129553701796No Hit
ATATCCAACTCTTTGAACCAGACGTCTGCACCCTTTTTCCTGATATACTG31040.1235603861580522No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA29000.11543979376879877No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA28610.11388732757673561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.1116979521776722No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA27980.11137949757417205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG27150.10807553106285815No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA27010.10751823550673294No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC26970.10735900820498287No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26860.10692113312517017No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC26420.10516963280591944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)