FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650077
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2149718.111877503936677No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA416741.572558080387853No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG247350.9333691058788103No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC206690.7799396017549679No Hit
GCCAGGCTCAGTGGCTCACGCCTGTAATCCCAGCACTTTTGGAAGCTGAG204220.7706191178595942No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA149660.5647383076038922No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA148780.5614176493739617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136440.5148529646497064No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC136190.5139095958343852No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT134550.507721096405878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113700.4290441372080887No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT111760.421723595201196No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG108100.4079126757448935No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC105870.39949782591222827No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA90960.34323530976647093No Hit
GAGCTCCTTGTCGTCGTCGTCCTTGTAGTCGATGTCGTGGTCCTTGTAGT86340.32580185405933487No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC77400.2920669852234482No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC75570.28516152549529694No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG68640.25901134193459285No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA68470.2583698511401744No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC62350.23527618254111107No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC57270.216106928213784No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT56760.2141824558305287No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC55340.2088241209595042No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA52520.19818292072268087No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG51370.1938434241722033No Hit
CTGTAATCCCAGCTACCCAGGAGGCTGAGGCAGGAGAATGGCTTGAACCC51000.1924472383255279No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC50390.19014541841614413No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG47160.17795709332219403No Hit
CTGTTTGGGGAGCACACTCTTCCATTATGCTTGGCACATAGATCTCCCTG46820.17667411173335718No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46250.1745232308344248No Hit
GACTACGGGCAACCGCAAGGCGGAGCTGCGGCTGTGCAACAAGCTGGTGG44540.1680705881376277No Hit
GGCCAAGGTGGGTGGATCATGTGAGGTCAGGAGTTTGAGACCAGCTTGGC43630.16463672564985846No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42370.15988214682063956No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC41720.15742938790080438No Hit
GGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAAC41360.15607093680674186No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC41140.15524077224925917No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT40660.15342950412384243No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC40070.15120315371968437No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC38220.14422222448630737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.1417694655664722Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37520.141580791803408No Hit
ATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG36720.13856201159438009No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC36620.1381846640682516No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA33300.12565672620078586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32960.124373744611949No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32340.1220341899499524No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT32330.12199645519733954No Hit
CCCCAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTG31940.12052479984543844No Hit
TTTTCTAGTAATAGCCACGACCAATTTATTAACAGTCAGGGCCTATCCTT31070.11724187636812063No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA31070.11724187636812063No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA31070.11724187636812063No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG30980.116902263594605No Hit
GCCGGGGACCTTGCCAACCCTCCCAAGAAGTTCAGAGACTGCCTTTTCAA30530.11520419972702681No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA30320.11441176992215699No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC29970.11309105358070727No Hit
CTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACT29840.11260050179674025No Hit
GTAGAGATGGGGTTTCACCATTGTGGCCAGGATGGTCTCCATCTCCAGAC29160.11003453861906654No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA28500.10754404494661854No Hit
GGCCAGCTCCACAGGTCCCTCGCCCCTGTCTGTGGCAGGATTCCCAAGGC27670.1044120604797521No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA27610.104185651964075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG26980.10180836254946553No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC26680.10067631997108008No Hit
TTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTT26600.10037444195017729No Hit
ACATCAATCTGACTGATTTGTTCTTTGAAAACATGAAATCGGGTCCCGCA26600.10037444195017729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)