FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710018
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT459022.684299229598753No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC156830.9171248489781979No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA124350.7271853278737417No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA99860.5839704611296489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96000.561397599323516No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG95750.5599356264086108No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT88270.5161933967946536No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC87400.5111057310507843No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84130.49198312532382704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80940.4733283509296394No Hit
CATATGGAAGCATTGCATTGATTGTCTTATATTTCAAGTTAAGGTCCAAA72460.4237382296560621No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG64380.37648726504633284No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA62110.3632125509789955No Hit
CTTCCAAGTTGTGGCTTTGGCTGCCACAATGTCCTCTCATTTATTGAGGT62030.36274471964622595No Hit
GCGGAGCCAAGCCCCTGCACCGGTGGTCCAGGCCCTTGTTCCCCAAGGCG62030.36274471964622595No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA58830.34403146633544207No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC55340.3236223244433684No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG54960.32140012561271286No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC54670.31970423703142303No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC45660.26701473317824725No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT39450.2306993259720073No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA39050.2283601693081593No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC37370.21853571131999783No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG36580.21391587690889804No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36440.21309717207655127No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC36380.21274629857697402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.21163519916164625Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG35790.20929604249779826No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA35260.20619665991819966No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31750.18567056019293365No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACA30680.1794133161171403No Hit
CACTTGAAGTTTATGTTGCCTGCATGGACAGCAGTCATTTCTGCGGCTAG30050.17572914437157972No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29090.17011516837834456No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA28550.16695730688214977No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC28210.164969023717879No Hit
GGTATGATATTAAAATAGCAACTAGAGTGTACTGAGTGGACACTGTGCTC26160.15298084581565807No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26010.15210366206671508No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC25940.1516943096505417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25850.1511679994011759No Hit
TCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGCTGTCT25610.1497645054028671No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG24920.14572946015772933No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA22740.13298105633975782No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC22630.13233778825719963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21690.12684077009715689No Hit
CTTTTCTGCTAATGAAGACAGATGTGGAAACGCCAGCCCGGTGCCCTTGC21500.12572967068182908No Hit
TTTTTGTCCTCCCCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTG19750.11549586027749414No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19750.11549586027749414No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19590.11456019761195496No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT19470.11385845061280056No Hit
TGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCG19180.11216256203151079No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT19170.11210408311491458No Hit
GCCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTT18900.1105251523668172No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAC18870.11034971561702858No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG18580.10865382703573881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18290.10695793845444902No Hit
AGATTAAGTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGC17970.10508661312337063No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA17350.10146092029440627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.10122700462802146No Hit
GTTTTTGGAGATGACTGAACAGATGAAGGCATCAGATGCCTTCATCAGCT17250.10087613112844426No Hit
GTTTTTTGGAGATGACTGAACAGATGAAGGCATCAGATGCCTTCATCAGC17130.10017438412928986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)