FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036247
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG624853.068635583011295No Hit
GGGACATGTTAACCCTGAACTGAAACCACTTTTTGGTGGCGGATGTTCTA200690.9855877012955698No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC163340.8021620166905096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138430.6798291169980852No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT136850.6720697439947119No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA133580.6560107884750721No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA119390.5863237613118644No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT114910.5643225011504007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110610.5432052201918529No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC109480.5376557951957694No Hit
CTTCCTATTTGAATACCCTTTCTTTCTTTCTCTTGCCTGATTGCCCTGAC92940.45642792843893687No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT88610.4351633176132365No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG83760.4113449890902233No Hit
GCTGAAGGTGTCTGCACACATCACCGAGCAGCTGGGCATGGCCCCAGGTG83100.4081037320128649No Hit
GACCAACATGGAGAAAACCCATCTCTACTAAATATATAAAATTAGCTTGG77700.38158435592538625No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC74090.36385566191134966No Hit
CAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGA68610.3369434061781306No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC64220.3153841356181249No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG62240.30566036438604943No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA61680.3029102068658665No Hit
TCCCTGCCCCCTCACAGGGCCAGCAGCGTGGGTGAGCTGAGCGAGTCAGA54590.26809124826212144No Hit
CATTCATTCTTCAGACTTATTTCTTACCTTGGTTGGCAACAGCAGCGGCA53490.26268915313319063No Hit
TCAGGAAGCTATCTCGGCTCTTAGAGTGCTTAATGTGCTCAATACGCACA50950.2502152243809322No Hit
CCCGAGTAGCTGGGATTACGGGCGCCCGCCACCACGCCCGGCTAATTTTT48700.2391654843444828No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT48410.23774129562867374No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC46960.23062035204962855No Hit
TCTTAATGCCTCGCGGTTAGCGTTGACGATTAGGATGGATTATACGCTCA43410.21318631776989727No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG43410.21318631776989727No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC42750.20994506069253877No Hit
GGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCC40320.1980113414531734No Hit
AGACGAAAGTGATGCTTTCCATGACAACCTTAGGTCTCTTGACAGGAATC38640.1897608688926245No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38400.18858222995540325No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC38390.1885331199996857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.18244348549070913Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37040.18190327597781605No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36750.180479087262007No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG36410.1788093487676102No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA34530.16957667709271027No Hit
TCCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT34170.16780871868687836No Hit
GGCGAGCATAGCACTTGCGGCAGATCATCTTGTCGCAGTTGTATTTCTGG30790.15120955365434546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30140.14801740653270454No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG30020.1474280870640939No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA29350.14413772003101785No Hit
GATTACAGGTGTGAGCCACTGCAACCGGCCATGACTTCGTTTCCTCTCCC29070.14276264127092636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.1333335297731562No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT26930.13225311074737003No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA26860.13190934105734717No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26450.1298958328729275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG25500.12523038707975997No Hit
GTATAAAGTGAATCATGATAAAAGTAAGAAACATAACTTCTTGTTGGTAT25160.12356064858536316No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25020.12287310920531744No Hit
GGACGGGTGACCAAGCGCAGACGTGGGTGACACGTGGCCCCGCTCTCTGG24590.12076138110946265No Hit
CTGGTATCATTGCCATTTACGGTTTAGTTGTTTCCGTTTTGGTTTGTTAT24010.11791300367784457No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24000.11786389372212702No Hit
GCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACC23970.11771656385497437No Hit
GATTAAGACTAGAGTGGCAGGTATTAGGGATAATATTCATTTAGCCTTCT22550.11074295014308186No Hit
CACCCAAGCTGGAGTGTAGTGGCACAATTAGGCTCACTGCAACCTCTGTC22500.1104974003644941No Hit
CTCTTGAGATTTATTCTTGGAGGACAGAGCAAAAGTCCACTCTTCAGCAG22200.10902410169296749No Hit
GCTAAAGAGCACGTAGCATGCCAGCACCATGGTAATCCCCGAGATGCTCC22180.1089258817815324No Hit
CTCACAGCTTGTGCAATGGCATCCCTGTCGATACCAAACATCTTCAGCCT21800.1070597034642654No Hit
ATCTTTACGTCGAAAGTTGGGGCGCCGTCCGTCTGCTCGACGCTGACATC21710.10661771386280741No Hit
CTCCCGAGTAGCTGGGATTACAGGCATGAGCCACCACGCCCAGCTAATTT21010.1031800169625787No Hit
GGGATTGATGGGAAGGGACAGATGTAGTTGGCAACCATGGTTAGCATCGG20690.10160849837961702No Hit
GGAGAAGCAAGCAGGCGAATCGTAATGAGGCGTGCGCCGCCAATATGCAC20670.1015102784681819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)