FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122071
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGCTTTTAAGGCTGCCGTTTTCTCAGACAGCCTGTGTCCTGATGCCA18021.476190086097435No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15281.251730550253541No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12781.046931703680645No Hit
CCTCTGGAGCCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG6660.5455841272701952No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG5930.4857828640709096No Hit
TAGATGTACTCAACACAAACTTTATCCCAGCAAAAGCACAGACTTGAGTC5720.4685797609587863No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5340.4374503362797061No Hit
AAATGGATATTCAAAATACCTTAGTGGCTGGGTGCAGTGGCTCACACCTG5050.41369367007725016No Hit
GCAGCGTCAGATGTGTATAAGAGACAGACGCAGAGTACATGGGAACTAGG4330.354711602264256No Hit
TGTCTCTTCACTGCTTGCTCTTGGTCTCTGCCCCTCTGCTGCCTTTGCAT4250.34815803917392335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.34733884378763175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.3366893037658412No Hit
AGGCGTGGTGGCAGGCACCTGTAATCCCAGCTGCTCAGGAGGCTGAGGCA3980.32603976374405064No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3490.28589918981576296No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3420.2801648221117219No Hit
GAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGT3160.25886574206814067No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGT2980.24412022511489215No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA2900.23756666202455948No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT2820.23101309893422678No Hit
CACTAGAATGCAGTGGCTTGATTTTGGCTCATTGCAACCTCTGCCTCCCG2810.23019390354793523No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC2810.23019390354793523No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2660.21790597275356144No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG2660.21790597275356144No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG2650.2170867773672699No Hit
GGTGCGCAGGATCGCGAGAAGGAAAGCGAGAACTAGCTGGGTAATGTTAG2440.1998836742551466No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC2370.1941493065511055No Hit
CCCGAGAGTACCGGCAAGTCGACCGTCGGCCCGCGCCTGGCCCGGGCTCT2340.19169172039223076No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT2320.19005332961964758No Hit
AGCTAAGACATGTGGAGAGAGTCTCCAGGGCCTGTGCAGATGTGGGATAT2290.18759574346077282No Hit
GAATTACAGGTGTGAGCCACCAAGCCCGGCTAATCCCAGTACTTTCAGAG2070.16957344496235796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.16711585880348323Clontech SMART CDS Primer II A (100% over 21bp)
CTTCGGGAGAGCAGGAGGAAGCTGGCCCCTCGTCTTCCCAAGCAGGACCC2030.16629666341719163No Hit
GCCTCATCATCGCTTACTTCTTTATCACGTTCCTTCTCCACAAAAAGAGT2020.16547746803090005No Hit
ACACGGCACATGCTTTCCAGCAAATGCGCCCGGCCCGCAGGCGGGTCGTG1920.1572855141679842No Hit
GGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAAC1920.1572855141679842No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT1910.15646631878169262No Hit
TGGTGCTTTTAAGGCTGCCGTTTTCTCAGACAGCCTGTGTCCTGATGCCA1870.15318953723652629No Hit
CGAAAGCGGCGATCGACATCACCGCGCTGGGCAGCGTTCCGGTGCTGATC1820.14909356030506837No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC1810.14827436491877677No Hit
GATCGATTGGGAAGAAGTTGAAATATGCCAAGCTGTGTAACAGCTAAGTT1730.1417208018284441No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC1730.1417208018284441No Hit
CTCCGGAGCTGGGTGCTGACAGCAGCCATGACCTTGTTGTTGCCATGGAC1710.14008241105586094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1660.135986434124403No Hit
GACTACGAGGCGTCATCCTCCCGCTGCAGCAGCGCGTCCCCGGCCGGGGA1640.13434804335181985No Hit
CGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGG1640.13434804335181985No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC1610.1318904571929451No Hit
GGCTTCCTCCACAATCCTCTTTTCCAGCTTCAGATCCAGGACCAGGACAT1580.12943287103407033No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA1580.12943287103407033No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT1580.12943287103407033No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA1520.12451769871632083No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC1490.12206011255744607No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT1480.1212409171711545No Hit
GCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGG1450.11878333101227974No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1430.11714494023969657No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT1420.11632574485340498No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT1420.11632574485340498No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGTCGACCGGCGATGCG1410.11550654946711339No Hit
GTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCG1410.11550654946711339No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA1380.11304896330823866No Hit
GGCCCTGCAGCAGCAGGCGGACGAGGCGGAAGACCGCGCGCAGGGCCTGC1350.1105913771493639No Hit
GGCAGGGAAGGGGAGGCAGCCGGCACCCACAAGTGCCACTGCCCGAGCTG1330.10895298637678072No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT1320.10813379099048914No Hit
GTACATGGGACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAG1320.10813379099048914No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG1310.10731459560419755No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC1290.1056762048316144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.1048570094453228No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA1270.10403781405903122No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG1260.10321861867273964No Hit
AGATGGAGAACCACAGACTTGTAAAAGTATGAAAGGATCAGAACTTAAAA1250.10239942328644806No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG1230.10076103251386488No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)