FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840484
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG901614.898765759441538No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA204771.1125877758241853No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC200941.0917780323001993No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA155850.8467881274708174No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT136940.7440434146670115No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC121500.660152438163005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117880.6404836988531277No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG115890.6296713255860958No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT108420.5890841756842222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100810.5477363563062759No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG87730.4766680938274932No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC76980.41825954477191873No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC73660.40022081148219707No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA70130.38104107397836656No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG62840.34143192768858627No Hit
GTTCCATCCCAGGAGCCTGAGAGGGCAAACTGGCCATCTGAGGAGATAAC60570.32909821546940915No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55160.2997037735726037No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC53800.29231441294789845No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC52700.28633772420732806No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC52520.28535972059523473No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49330.26802732324758055No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46050.25020592409387965No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGA44050.23933921729284252No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42870.23292786028023066No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA42730.23216719080415804No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA38850.21108577961014602No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36620.1989694015269896No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA34780.18897203127003548No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.18571201922972436Clontech SMART CDS Primer II A (100% over 21bp)
GGCTCAGAGCCTGATTGACAAAACAACGGCTGCATTTCAACAAGGAAAGA32490.17652965198284798No Hit
GGTCTCGATCTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAAAGTG32400.17604065017680132No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA32230.17511698009871315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31740.17245463693245908No Hit
TCTTAATGCCTCGCGGTTAGCGTTGACGATTAGGATGGATTATACGCTCA29320.15930592170320415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27680.15039522212635373No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26880.14604853940593887No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC24440.1327911571086736No Hit
TCCCAGCACTTTGGGAGGCCGATGCGGGCGGATCACCTGAGGTCAGGAGT23960.13018314747642468No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC23760.12909647679632097No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC22670.12317412158975574No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG22490.1221961179776624No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC22230.12078344609352756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.117686434655232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21640.11757776758722163No Hit
CTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAG21220.11529575915900382No Hit
GTGTGTAATGTGGCCCTTCCTTCTCAAAGGGGAACAGGTTAGGATCCCGC21060.11442642261492086No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA20950.11382875374086382No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20490.11132941117662529No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA20040.10888440214639192No Hit
CCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGG20000.1086670680103712No Hit
ACTGAGCTCTGTCATCTGTTTTGGCTTTTATGCATACTGTGATTTGGGAG19890.10806939913631415No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT19660.10681972785419487No Hit
CAAATGTGCTTTCTCCTTGTCTTTTCTCTCCATGCACCGTTTCACTTCCT19200.10432038528995634No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT19170.10415738468794078No Hit
CTTTGAGACGATGCCATCGGCCTTGGCCAATCGGAGAATGGAATCATCTG19160.10410305115393559No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA18690.10154937505569188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)