FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733991
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT697754.023953988227159No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC158330.9130958580523197No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA148240.8549063980147532No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA126680.7305689591237786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115510.6661510930564231No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT104250.6012141931532516No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC98820.569899151725701No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG92420.5329900789565806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89990.518976165389555No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT86290.4976381076949073No Hit
GCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATG84200.4855849886187414No Hit
GCTCTGTCACCCAAGCTGGAGTGCAGTGGCGTGATCATGGCTCACTCCAA82010.472955165280558No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA73250.4224358719278243No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC69100.39850264505409777No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC64400.3713975447392749No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG59860.34521517124368006No Hit
CACCAATTGCAAGAACAGGATTTTGACAAATTACTCAAGGTAGGAAGCCC59520.3432543767528205No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC58730.33869841308288223No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGG56890.3280870546617601No Hit
CGCCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTG56190.3240501248276375No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG54970.3170143328310239No Hit
AGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAAC51360.2961953089721919No Hit
GATGAACATGGCGGTAGAAACGGCCGAACCGTAACCAAACTGCAATGTCG50050.28864048313976254No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA47540.2741652061631231No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC47500.2739345244583161No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT46060.26562998308526403No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC42800.24682942414349326No Hit
GTGGTATCATTGTTTTGATTTGCATTTTTCTAATCATGTTGAGCCTCTTT42470.24492630007883548No Hit
ACGCAGAGTACATGGGGGGGGTTGTGGAGTGGATGCTTTGGCAAGATGGC42220.24348453942379172No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG42180.2432538577189847No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC41610.2399666434254849No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA39290.22658710454667874No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG37720.21753284763300385No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG34800.20069308318209264No Hit
CCTTTTCAGGCAGAAACACAGAGACCCAGAAAGGGAAAGTGGTTGGACTG32740.18881297538453198No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31930.18414167086219016No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG31370.18091212699489212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29920.17254991519563828No Hit
GTCCATGCTGATCGTGTTGTAGCTGCCGATCGAGGCGGTCGCGCGGCTCT29670.1711081545405945No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.1689743487711297Clontech SMART CDS Primer II A (100% over 21bp)
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT28860.1664368500182527No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC24890.14354169081615764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24660.14221527101351736No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG23800.1372556143601668No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA22820.13160391259239523No Hit
ACTGACAACGAAGGCCGCGCCTGCCTTTCCCATCTGTCTATCTATCTGGC22340.1288357321347112No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21470.12381840505515887No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG21420.12353005292415012No Hit
CCAGCTCCTTGCACCTCTGGGCCACATCCTGGGCAGCCAACATAGCAGCA21180.12214596269530811No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC20840.12018516820444859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20700.11937778223762407No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20330.11724397646815929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.11637892007513304No Hit
GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCT19900.11476414814148402No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA19830.11436045515807176No Hit
GATTACAGGTGTGAGCTACTGCATCTGGCCTTAATTTTTACTTTAAAAAT19590.11297636492922974No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT19290.11124625214317721No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19270.11113091129077372No Hit
CTCTGGCAGCCTTGAGAGCATCAGGTGGATGCATGAGCCGGAGCCCGCAT18920.10911244637371244No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC17960.10357608545834436No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT17910.10328773332733561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)