FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911954
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC619443.2398268996011415No Hit
GATTGCCCAAGGACTTTTTTTCTCTAAATGAAGCCCCATCTCAGGTGGAT155800.8148731611743797No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC153500.8028435830569145No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA141630.7407604994680834No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT124150.6493357057753482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122800.6422748664455316No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA116330.6084351401759666No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA107460.5620428106533945No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC102690.5370945116880428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98130.5132445655073292No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG93160.48725021627089354No Hit
CGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCG88190.4612558670344579No Hit
CTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGA86790.4539335151368704No Hit
TGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCAC86540.4526259522980155No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT85870.4491216838898844No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA77670.4062336227754433No Hit
CTATTACATCTTTGCTATATGCAAAATACCCTTAACTGTAGTTAAAAGTT73220.38295900424382595No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC68560.35858603292757046No Hit
CATCGACCCCGAACATCTCTTCATCGCCCTGGCCGGTGACCCCGATCATG65850.3444120517543832No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG63450.33185944850137605No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG62680.32783215495770296No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC59880.31318745116252794No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC46690.24420043578454292No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT44950.23509979842611275No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG43480.22741132893364588No Hit
CTGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAACCT42620.222913312767985No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC40790.21334195278756707No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38440.2010508621023309No Hit
CCCCAGCAGTGTCCGGGCTCTCCCGGCAGGTGCGATGCTTCAGTACCTCT37010.19357160266408083No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC36790.19242094736588852No Hit
GTAGTAGAGATGGGGTTTCACCACGTTGACCAGGCTGGTCTCGAACTCCT36540.19111338452703358No Hit
CTTTTCTTCTTGTTGTTTTGCCTCTTTTTCATACATCACTTTTTCTTTGA34850.1822742597363744No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC34300.1793976214908936No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATT33550.17547493297432887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.17469039527101593Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32370.1693032363749337No Hit
TTAGAATCCCAGGCCCTTGCAGGTAAGTCTGACAGTTTCAGTGAGCTCTG32210.16846639615806658No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG30870.16145785934180426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27490.14377960976048587No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC26420.13818324081018687No Hit
GTCCGGCCTACAGAGCCAGCCCCCTGCTGGCCATCGGGCAGACGCTGGCC25910.13551581261892284No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25880.13535890507826026No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC24420.1277227380993476No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA23690.12390465460989124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.12191715909483178No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG22830.11940663844423034No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA22630.11836058817314642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22520.11778526052405028No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA22480.11757605046983349No Hit
CGGCTGCTTGGGAGGCTGAGGTGGGAGGATCATTTGAGCCTGTAAGGTTG22100.11558855495477402No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21120.11046290862646278No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC21100.11035830359935439No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20590.10769087540809036No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT20440.10690633770477742No Hit
AGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGC20330.10633101005568124No Hit
CTTTCTTTGGTTGGCTAGAATGGAGCTACTAATGCTTATTTGACCCTTGA20310.10622640502857285No Hit
GTATCAACGCAGAGTGCATGGGACGGCGAGTACTGTCTTGCTGGGTCTTT19220.10052543105116545No Hit
GTATAAGAAGGGCAAGGATTCTTTGTATGCCCAGGGAAGGAGGCGCTATG19210.10047312853761126No Hit
CCATGTTTAAGAGCTGTAAAGGGGGGCCAGGCGCAGTGGCTCACTCCTGT19200.10042082602405705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)