FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894993
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC303871.6035415434252263No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC146210.771559578320342No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA114370.6035378494801829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114280.6030629136888632No Hit
GTATTTTGCCTTGGATCTCCTGATGGAGAATGGGGAGTGCCGTGGTGTCT108670.5734585826966115No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC101200.5340389120170892No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT98050.5174161593209051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88880.46902547924979143No Hit
GTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC86370.4557800477363241No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT80900.42691450575279166No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA68610.3620593849159337No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC65990.34823347632418694No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC63310.33409094387156046No Hit
ACCTAGCAAGTACCGCGGCAGCTAACCTGGGAGAACAACCCAACCAACAG61960.3269669070017673No Hit
GATCTACCTTTACTGGGGAAAAACAACCATTACTAAGCCTTAGGGGAAAT61220.323061879384251No Hit
GCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCTGGCAGATCAC60640.32000118206241396No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA57000.30079266783571235No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG52090.2748822818870571No Hit
GGTTTATTCCCTGGTGCCACCAGCCTTCCTGTGGGCCCCTTAGCAATGTC50060.26416984126062737No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA49500.26121468522575014No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG48070.2536684832081174No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG47980.2531935474167979No Hit
CACACTCACTAAAGGAAAGAATGCATAGCACAGCCTGAGATGAATAAATG47920.2528769235559182No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT43560.22986892299866013No Hit
CACCCAAATACAGGATGATAGTTCGTGTGACAAACAGAGATATCATTTGT41620.21963141816354997No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG40900.2158319318329936No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA40240.2123490693633169No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC40080.21150473906763773No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC36110.19055479360609776No Hit
GCCGGGCGTGGTGGCGGGCGCCCGTAATCCCAGCTACTCGGGAGGCTGAG35050.1849611053972231No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG34700.18311413287542488No Hit
TCTCTGGAAAGAACAGAAGAGTGACCAGTTCCTCTGTCAGTTTGGTCTCA33890.17883971075354896No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC33180.17509299506647252No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC32310.17050194908371694No Hit
ATCTGGGACCCACTGCCCCACCCAGAAGTGGCCCAGTCCTGGGGGCAGGG31880.16823281141407911No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA31100.16411670122264305No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA29500.15567339826585114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29500.15567339826585114No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA29280.15451244410929224No Hit
CGTGAGCCACCATGCCTGGCTTAGTTTTTGTATTTTTAGTAGAGACGGGG28610.1509768109961356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.1493936916917371Clontech SMART CDS Primer II A (100% over 21bp)
GGCATAGTCAGGGAGCATGAGTAGGAGATTCCAGTGTGCAGCCAAAGTTG28150.14854936139605793No Hit
CCAGCAGATTTGGGGCTGGGGACACTGGGCCGGGAATCAAGGGTCTGGAC26920.14205857224802412No Hit
GCTCTGCTTCTGTCTTCCACTCAACAGCATTGACAGCCCCTCCCAACTAT26570.1402115997262259No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC26500.13984220522186627No Hit
GGCTGGAGTGCAATGGCACGATCTCGGCTCACTGCAACCTCCACCTCCCG26160.13804800334354797No Hit
CTGACTTCAGCGCTCCCACTCTCGGCCGACACCCCTCATGGCCAACCGTT25020.1320321499868337No Hit
GGACTCAGAGGCCGCCATCAACCGCCAGATCAACCTGGAGCTCTACGCCT24910.13145167290855428No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC24630.1299740948911157No Hit
GCTTGGACCGACTATTCGACTTCGGGCTATGTCACCACCTGGAAGGCGGG24390.1287075994475969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24300.12823266365627736No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTAGTAGCCGTT24020.12675508563883878No Hit
ACACCTGAGGAGGCCAAGTACAAGTTGTGCAAAGTGAGAAAGATCTTTGT23670.12490811311704055No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG23590.12448594796920096No Hit
GTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGC23520.1241165534648413No Hit
GGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC23170.12226958094304306No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG23030.12153079193432377No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA22860.12063369099516463No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC22660.11957827812556564No Hit
CAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGT22560.11905057169076613No Hit
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT21990.11604264501240902No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21310.11245424125577244No Hit
CCCTTGGCGTGGAGCCAGGGAGCGGCCGCCTGCGGGCTGCCCCGCTTTGC21290.11234869996881254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.10928800264697548No Hit
ACCATCCTCCAGACCCCAGAATAGTAGATCCACCTACAGCTTGCAACATG20460.10796873655997674No Hit
GACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGT19920.10511912181205947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19910.10506635116857951No Hit
GCCTCCTCTGTTATTTTCTCTAAACTGTAAGCTCTGAGAGATGAATTGTG19860.10480249795117977No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG19790.10443310344682012No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19290.10179457127282264No Hit
CTACTAATGGATAGAAAATGATTGGGGATTGGAGCTACTTCACATGGAAT19260.1016362593423828No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA19010.10031699325538405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)