FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995940
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG52049426.077637604336807No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA678313.3984488511678705No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG560462.808000240488191No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG321101.6087657945629628No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA311021.5582632744471276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176160.8825916610719761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144230.7226169123320341No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG117130.5868412878142629No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA112130.5617904345822019No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC107610.5391444632604186No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT95210.4770183472449072No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG87220.43698708378007356No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA74200.3717546619637865No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA70870.3550707937112338No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC69000.34570177460244295No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC64030.3208012264897742No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61830.30977885106766734No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC53150.2662905698568093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.26253294187200016Clontech SMART CDS Primer II A (100% over 21bp)
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG50020.2506087357335391No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG46270.23182059580949327No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT45150.2262092046855116No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA44460.22275218693948717No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA44030.22059781356152988No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43390.21739130434782608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG38850.19464512961311461No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT38800.194394621080794No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA38030.1905367896830566No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC36590.18332214395222302No Hit
GCAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA34620.17345210777879094No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAA33670.16869244566469935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.16328146136657415No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA32560.16313115624718177No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31960.16012505385933445No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA31950.16007495215287032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG28290.14173772758700162No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC26450.13251901359760315No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC26220.13136667434892832No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC26160.1310660641101436No Hit
GCAATCGCAGGCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCAC25740.12896179243865047No Hit
GCGAAGGCTTCCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG25280.12665711394130086No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24870.12460294397627182No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC24840.12445263885687947No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT23500.1177390101906871No Hit
GCGAAGGCTTCCCCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG23010.11528402657394511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22620.11333006002184434No Hit
TCCATGAGGGTGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20560.10300910849023517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)