FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293251
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG66232.258474821910241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55301.8857565703100758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49741.696157898864795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.47297366419892856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.44501127020879727Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC11450.39045050144756543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11100.3785153332810459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10990.37476428042871124No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAA10450.3563500209717955No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA9420.3212265260817525No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9410.3208855212769948No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC8240.28098795912034397No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT8120.2768959014632516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7580.2584816420063359No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7510.256094608373032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7100.24211341137796633No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA6910.23563432008757004No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT6760.23051924801620452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6530.22267613750677748No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA6180.210740969340258No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAA5760.19641876754043466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG5500.18755264261673446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5200.1773224984740035No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5170.1762994840597304No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC4910.16743335913603022No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAA4790.16334130147893783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG4320.14731407565532598No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA3980.13571991229356423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3970.13537890748880652No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC3950.13469689787929112No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAA3950.13469689787929112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3880.13230986424598723No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC3840.13094584502695644No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT3830.13060484022219873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.12310273451752934No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.11423660959382917No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA3230.11014455193673678No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA3190.108780532717706No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG3110.1060524942796444No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3060.1043474702558559Clontech SMART CDS Primer II A (100% over 26bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC3020.1029834510368251No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG2960.10093742220827892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)