FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727316
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA462006.3521220487381No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA133491.835378294991448No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA65920.9063460724086916No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG65270.8974091041583026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49980.6871841125453035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.48438367917108927No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC32520.44712339615792857No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30020.4127504413487398No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT26170.35981609094258893No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24660.33905482623783884No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA23560.3239307261217958No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20850.28667044310863504No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19770.27182132663106545No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC18350.25229748829944615No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA14030.19290102238916784No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13490.18547646415038305No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC12190.16760252764960484No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12160.1671900521918946No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11890.1634777730725022No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11670.16045295304929358No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC11020.15151598479890446No Hit
ATGTTGGCCTTGAGAGAGATGGCACTGAGTTCATTCAGCTCCTGGTTGTT10470.14395393474088292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10390.14285400018698885No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC10050.1381792783329392No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10030.1379042946944657No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC10010.13762931105599216No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT9960.13694185195980837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.13185465464804844Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT9570.13157967100957493No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT9310.1280048837094193No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9290.12772990007094578No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9270.12745491643247228No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC9180.12621749005934146No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG9090.12498006368621067No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9020.12401762095155337No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT8870.12195524366300206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.1205803254706345No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG8270.11370573450879673No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA8240.11329325905108645No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8220.11301827541261296No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA8060.11081840630482485No Hit
CATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG7960.10944348811245731No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7930.10903101265474703No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG7890.10848104537780003No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA7630.10490625807764438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7540.10366883170451358No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7510.10325635624680331No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA7300.10036902804283145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)