FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394778
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA749125.370890564663338No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA183121.312897106206149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118030.8462278584835723No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG93400.6696406166429353No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA84090.6028916429711395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.6013143310261562No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA77970.5590136925016024No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA47150.3380466282089336No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA45000.3226319887465962No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC44070.3159642609791666No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43510.31194928511920894No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC42070.30162506147931784No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT36740.26341109481222097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC34360.24634744740740103No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC32440.23258181588754626No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC30060.21551816848272629No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG29190.2092806167002921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26920.1930056252679638No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA26340.18884725741300765No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG25780.18483228155305004No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC23970.17185530600568694No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC23560.16891577010821796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.16253482633078525Clontech SMART CDS Primer II A (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.15385961063337678No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA20160.14453913095847512No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT19660.14095433108351293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18590.13328285935109385No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT18530.1328526833660984No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG18390.131848939401109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18030.12926788349113622No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG18030.12926788349113622No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17340.12432085966368842No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC16810.12052097179622849No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT16540.11858517986374892No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT16300.11686447592376707No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC15940.1142834200137943No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15680.11241932407881397No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA15380.11026844415383667No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT14920.10697042826887146No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT14230.10202340444142365No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC13960.10008761250894407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)