FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40600
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1047325.795566502463053No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9832.4211822660098523No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7661.8866995073891626No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG5651.3916256157635467No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG5591.376847290640394No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG5571.3719211822660098No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3800.935960591133005No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA3610.8891625615763546No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3120.7684729064039408No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA2790.687192118226601No Hit
CCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2290.5640394088669951No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC1700.41871921182266003No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC1540.3793103448275862No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC1530.3768472906403941No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1450.35714285714285715No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA1400.3448275862068966No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1260.3103448275862069No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC1230.3029556650246305No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG1160.2857142857142857No Hit
GCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT1150.2832512315270936No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1140.28078817733990147No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1080.2660098522167488No Hit
TCCATGAGGGTGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1000.24630541871921183No Hit
TCCCAGCACTTTGGGAGGCTCACGTGGGAGGATCACCTGAGGTCAGGAGT970.23891625615763545No Hit
ATTCAGGGAAGTCCAGGATGGCAGCTGAAGGCAGCAGGTTTAGCAGCCAA860.21182266009852216No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT840.20689655172413793No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC820.20197044334975373No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG810.19950738916256158No Hit
CCATAGCACATACCCTCTTGAACAGGCTCCTTTTGCTCCCATGTACTCTG790.19458128078817732No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA780.1921182266009852No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA760.18719211822660098No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG750.18472906403940886No Hit
TCCATGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT740.18226600985221675No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA700.1724137931034483No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT700.1724137931034483No Hit
TTGATATCTGGCTGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAG700.1724137931034483No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT690.16995073891625617No Hit
ACCTCTCAAAGTGCTGGGACTGTCTCTTATACACATCTCCGAGCCCACGA680.16748768472906406No Hit
GTCTTTCTCTGTTGCCCAGTCTGGAACACAGTAGCACAAACACAGTTCAT650.16009852216748768No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG640.15763546798029557No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATAAACATCTCCGAGCCCACGAGACA630.15517241379310345No Hit
GCGAAGGCTTCCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA610.15024630541871922No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC600.1477832512315271No Hit
ACATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA590.14532019704433496No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC590.14532019704433496No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTCTGTCTC590.14532019704433496No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC570.14039408866995073No Hit
GCGAAGGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT570.14039408866995073No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA550.1354679802955665No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC540.1330049261083744No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA540.1330049261083744No Hit
ATGCCACAGAATTGTGTACTCTAAAATGGTTTAATTGCTGTGCATGGTGG540.1330049261083744No Hit
TCCATGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG530.13054187192118227No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT530.13054187192118227No Hit
TTGATATCTGGCTGTCCACTGTCTCTTATACACATCTCCGAGCCCACGAG510.12561576354679801No Hit
GTGGATTTTCTCTTGAGTCTCTGTCTTCTTCAGTTTCGACTTATCGAATT510.12561576354679801No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT500.12315270935960591No Hit
AAGTTGATAGCCGCCGAGACATGCAGCACTCCTGCTGCCTGGAAAATGCG500.12315270935960591No Hit
TTTTGAGACAGAGTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCAC490.1206896551724138No Hit
CTTCCACAGCAAACAAAATCCACTTTTAGAACGACAACAACCCAGGGGAA480.11822660098522167No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC480.11822660098522167No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC470.11576354679802955No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG460.11330049261083744No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC450.11083743842364532No Hit
GCAAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA440.1083743842364532No Hit
TCTGTCTTCCACTCAACAGCATTGACAGCCCCTCCCAACTATGTCCTCAC420.10344827586206896No Hit
CCTAATTACATAAAATGGAATACTGATAGCTACTTGCTAGAGGTGGCCTG420.10344827586206896No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG410.10098522167487686No Hit
GATAGGAGAAGTAGGACTGCTGTGATTAGGACGGATCAGACGAAGAGGGG410.10098522167487686No Hit
GCGAAGGCTTCCCCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA410.10098522167487686No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC410.10098522167487686No Hit
ACCATAGCAGATACTTCCACTACACCTTTTTGGTTTTGTGACATTGGCCT410.10098522167487686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)