FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295705
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA69320.5349983213771653No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA59700.4607530263447313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41280.31859103731173377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC39160.3022292883025071No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA37890.292427674509244No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA36430.2811596775500596No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC34970.26989168059087526No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG34620.26719044844312556No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC31350.24195322237700714No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT27610.21308862742676765No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC27390.21139071007675359No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT27160.2096156146653752No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC27060.20884383405173246No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG26440.20405879424714732No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA26060.2011260279153048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.19209619473568443No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT24810.19147877024477022No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA22590.17434524062190082No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT21630.16693614673093027No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC20680.159604230901324No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC20580.15883245028768123No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20530.15844655998085982No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG20180.15574532783311015No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC19830.15304409568536048No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT19500.15049721966033935No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA19490.15042004159897507No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC19370.14949390486260375No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG19310.1490308364944181No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC18870.14563500179438993No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC18020.1390748665784264No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG17490.13498442932611976No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA17370.13405829258974844No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC17270.13328651197610566No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG16230.1252599935942209No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT16200.12502845941012808No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC16130.12448821298057815No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC15980.123330542060114No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA15730.12140109052600709No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA15580.12024341960554294No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA15530.11985752929872155No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA15480.11947163899190015No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT15460.1193172828691716No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG15340.11839114613280029No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT15070.1163073384759648No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15000.11576709204641489No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC14520.11206254510092963No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA14360.11082769611910118No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC14330.11059616193500835No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG14320.11051898387364406No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT14300.11036462775091552No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT14260.11005591550545843No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC14110.10889824458499428No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAC13980.10789492978725866No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG13840.1068144369281588No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA13720.10588830019178748No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG13510.10426756090313768No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG13110.1011804384485666No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATG13010.10040865783492385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)