FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132231
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1897714.351400201163116No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48743.6859737882947265No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35692.69906451588508No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT29532.233213089215086No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA20351.5389734631062306No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC19131.4467106805514591No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA17301.3083165067193017No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC15221.1510160249865766No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT13361.010353094206351RNA PCR Primer, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11410.8628838925819211TruSeq Adapter, Index 11 (95% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11090.8386838184691942No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC10230.7736461192912403No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT9630.7282709803298772TruSeq Adapter, Index 11 (96% over 26bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT9270.7010458969530594No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC8510.6435707209353329No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG7740.5853392926015836No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7320.5535766953286294No Hit
GCGAAGGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG5780.4371138386611309No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC5570.4212325400246538No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG5550.4197200353926084No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC5210.394007456647836No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG4830.3652698686389727TruSeq Adapter, Index 11 (95% over 24bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT4670.35316983158260923No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC4550.3440948037903366No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG4520.34182604684226847No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT4470.33804478526215487No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC4320.3267010005218141No Hit
GGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT4210.31838222504556424No Hit
GGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4040.305525935673178No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT4010.3032571787251098No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT3930.2972071601969281No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT3710.2805696092444283No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA3580.270738329136133No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC3340.25258827355158775No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA3290.24880701197147417No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG3190.241244488811247No Hit
GCGAAGGCTTCCTCTCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCA2690.2034318730101111No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC2660.20116311606204296No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT2640.19965061142999752No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT2600.19662560216590663No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG2570.19435684521783847No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA2360.17847554658136142No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2330.17620678963329325No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT2310.1746942850012478No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACAT2310.1746942850012478No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC2230.16864426647306607No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA2170.16410675257692978No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2060.15578797710067988No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA2050.15503172478465715No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT1960.1482254539404527No Hit
CTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCG1900.1436879400443164No Hit
GCGAAGGCTTCCTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1860.14066293078022551No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1840.13915042614818007No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT1810.13688166920011194No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC1790.1353691645680665No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGT1760.13310040761999833No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA1670.12629413677579387No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC1580.11948786593158942No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG1560.11797536129954397No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA1560.11797536129954397No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT1510.11419409971943038No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT1500.11343784740340766No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT1420.10738782887522592No Hit
CGTCAGATGTGTATAAGAGACAGTCCATGAGGGATTCCTCTCTGTCTCTT1390.10511907192715779No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT1380.10436281961113507No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC1370.10360656729511235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)