FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226554
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2331310.290261924309437No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA64042.826699153402721No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC35921.5854939661184528No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA31721.4001077005923532No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC25251.1145245725080997No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG23061.0178588769123476No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA21290.9397318078692056No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20960.9251657441492978No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC19410.8567493842527609No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG18820.830707027905047No Hit
GCGAAGGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC18380.8112856096118365No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA18330.8090786302603353No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT15560.6868119741871694No Hit
GGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA12590.555717400707999No Hit
GGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11720.5173159599918783No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT10220.4511065794468427No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC9360.4131465346010223No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC8670.38269021955030585No Hit
GCGAAGGCTTCCTCTCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCA8070.3562064673322916No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7400.32663294402217574No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT7320.32310177705977383No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT6780.299266400063561No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT6620.2922040661387572No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTGGCGG6520.28779010743575484No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT6190.273224043715847No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA6020.26572031392074297No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG5660.24983006258993443No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT5540.24453331214633156No Hit
GCGAAGGCTTCCTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5390.23791237409182797No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT4900.21628397644711636No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA4380.193331391191504No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT4220.1862690572667002No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT4210.18582766139639997No Hit
GCGAAGGCTTCCTCTCTGTCTCTGTCTCTTATACACATCTCCGAGCCCAC4090.18053091095279714No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT3950.1743513687685938No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC3900.17214438941709262No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC3890.17170299354679236No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTTTGGCGG3700.16331647201108787No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT3580.158019721567485No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA3530.15581274221598382No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA3280.14477784545847788No Hit
GCGAAGGCTTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT3270.14433644958817765No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC3200.14124667849607597No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGATGGCGG3160.13948109501487505No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACAT3130.13815690740397432No Hit
GCGAAGGCTTCCTCTCTGTCCTGTCTCTTATACACATCTCCGAGCCCACG3130.13815690740397432No Hit
TCCATGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC3030.13374294870097195No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGCGG2940.1297703858682698No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT2890.12756340651676862No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA2670.1178526973701634No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG2640.1165285097592627No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATCTGTCTCTTATACACATCTCCGAG2630.11608711388896245No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACCTGTCTCT2570.11343873866716103No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCCTGTCTCTTATACA2530.11167315518596008No Hit
GCGAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC2490.10990757170475914No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA2420.10681780061265747No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTATGGCGG2350.10372802952055582No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCCTGTCTCTTATAC2330.10284523777995534No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC2320.1024038419096551No Hit
GTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCT2320.1024038419096551No Hit
AGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCAC2300.10152105016905462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)