FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482117
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7526415.611148331214208No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA125832.609947377918638No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG84231.7470862881831588No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA60051.2455482797744115No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG37190.7713895174822709No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA35350.7332245077439709No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC25890.5370065772416239No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC24580.5098347496562038No Hit
AGCGAGACTCTGTCTCAAAACAAAACAAAACAAAACAAAAAACTATTCAA23290.4830777591331565No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG20910.4337121487107901No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC20280.4206447812460461No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18070.37480528585384876No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17790.3689975669806292No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA17560.36422694076334167No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT17360.3600785701396134No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT16850.34950022504910633No Hit
CATATATGGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGC16180.33560318345961665No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC15450.3204616306830085No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT14720.3053200779064003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.2841633877253862No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13340.2766963206026753No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13250.27482955382199753No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC13050.27068118319826934No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12430.25782123426471165No Hit
GAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGA12430.25782123426471165No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG12160.2522209339226785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.25159867832911925No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11700.2426796814881035No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGT11320.23479777730301982No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC10900.22608619899319046No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT10770.2233897580877671No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC10570.21924138746403882No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG10240.21239657593488717No Hit
GTAGAGACAGAGTGTCACTATGTTGCCCAGGCTGGTCTCAAATTCCTGGG9690.20098855671963445No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA9240.19165472281624585No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGG9110.18895828191082248No Hit
CTCCTGGCCTCAGGTGATCCCCCCACTTCTGCCTCCCAAGGCACTGAGAT9030.18729893366133116No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC8510.17651317003963768No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8130.16863126585455399No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG8090.16780159172980832No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA8000.16593482494913062No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7760.1609567802006567No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC7710.1599196875447246No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7290.15120810923489528No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6880.14270394945625234No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6840.14187427533150668No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA6830.14166685680032023No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG6800.14104460120676102No Hit
TGCTTCCGGATGACTGACCTCTCAAAGTGCTGGGACTGTCTCTTATACAC6760.14021492708201536No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACAT6560.1360665564582871No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA6400.1327478599593045No Hit
TGGTAGTACCCGGCTGCATCAAACGGGTCCTTCAGGTCGTAGAAGATGAT6100.1265253040237121No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG6080.12611046696133926No Hit
TGCCCCATCCTAGCTGGACTCATGGTTCCTAAATAACCACGCTCAGAAGC5930.12299918899354306No Hit
GCAATCGCAGGCACTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACG5680.11781372571388272No Hit
TACCACCTCTTCGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAG5580.11573954040201859No Hit
GTCAAGAACAGGGAGGAGCTGGGTTTCCGGCCCGAGTACAGTGCGAGTCA5480.11366535509015448No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC5270.10930956593523979No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCTTATAC5180.10744279915456208No Hit
GCGTTAAGAGTCCCCAAAGGAGGGAAGCCACCCTGCAATGGAATGGCAGA5120.10619828796744359No Hit
GAAGAGGACCATTTGAGTAGAAGAAGCAACACGAGTAGAGATTTGGCAGT5070.10516119531151152No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG5060.1049537767803251No Hit
GCAATCGCAGGCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCAC4890.10142766175015609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)