FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112396
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1263911.245062101854158No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA41803.718993558489626No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC17441.5516566425851455No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC15431.372824655681697No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15091.3425744688423076No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13671.2162354532189759No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11351.0098224136090252No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG9560.8505640770134169No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA8270.7357913092992633No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG7900.7028719883269867No Hit
GCGAAGGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC7860.6993131428164703No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC7800.6939748745506957No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6950.6183494074522224No Hit
GGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA5990.5329371151998292No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT5700.5071354852485854No Hit
GGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5520.49112068045126156No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC4420.3932524289120609No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG3960.35232570554112247No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC3920.3487668600306061No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT3620.3220755187017331No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTGGCGG3340.29716360012811843No Hit
GCGAAGGCTTCCTCTCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCA3290.29271504323997294No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT3120.2775899498202783No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA3090.274920815687391No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT3040.2704722587992455No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG2880.25623687675718No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT2830.2517883198690345No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT2740.24378091747037262No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC2710.2411117833374853No Hit
GCGAAGGCTTCCTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2480.2206484216520161No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2410.2144204420086124No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC2310.20552332823232144No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA2120.18861881205736858No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA2060.183280543791594No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT2030.1806114096587067No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT1990.17705256414819032No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA1900.16904516174952844No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTTTGGCGG1880.16726573899427027No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT1840.16370689348375386No Hit
GCGAAGGCTTCCTCTCTGTCTCTGTCTCTTATACACATCTCCGAGCCCAC1840.16370689348375386No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT1750.155699491085092No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGATGGCGG1550.13790526353251004No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA1470.13078757251147727No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT1470.13078757251147727No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACCTGTCTCT1450.12900814975621908No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA1450.12900814975621908No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGCGG1450.12900814975621908No Hit
GCGAAGGCTTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT1400.1245595928680736No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACAT1370.12189045873518631No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1350.12011103597992812No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC1330.11833161322466991No Hit
GCGAAGGCTTCCTCTCTGTCCTGTCTCTTATACACATCTCCGAGCCCACG1330.11833161322466991No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC1300.11566247909178262No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTGTCTCTTATACACAT1290.11477276771415353No Hit
TCCATGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC1260.11210363358126624No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGTATGGCGG1240.11032421082600805No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC1240.11032421082600805No Hit
GCAATCGCAGGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC1210.10765507669312074No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC1180.10498594256023346No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC1180.10498594256023346No Hit
GTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTA1170.10409623118260436No Hit
GTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCT1170.10409623118260436No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCCTGTCTCTTATACACATCT1160.10320651980497526No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG1150.10231680842734618No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCCTGTCTCTTATAC1140.10142709704971707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)