FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153833
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14310812.402834725649207No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA199911.7325730846664986No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG152571.322288407421178No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG83530.7239349195247493No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA76870.6662142615092479No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC50670.4391450062530713No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC46870.40621129747545787No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT41740.36175079062567983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.3547307105967675No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA39870.34554393920090687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39280.34043054757490904No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG38100.3302037643229133No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC35160.30472347384760184No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC34120.29571003776109717No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT34110.29562337010641926No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA30430.26372967318494095No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT29930.2593962904510445No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA29330.25419623117036866No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT25660.22238920190356834No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC24770.2146757806372326No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC23900.2071356946802527No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC23610.2046223326945927No Hit
CCATTCTTTCTGGAATTATGCATGATTCTGATTTCCCTTTCCAAGACCAG23520.20384232380249134No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG23440.2031489825650679No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA23340.2022823060182886No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC22110.19162218449290322No Hit
GCAATCGCAGGCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCAC18890.16371519968660975No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG18230.15799513447786637No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT18020.15617511372962986No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG17500.1516683956863775No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17490.15158172803169956No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17380.15062838383024232No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC16620.14404164207471964No Hit
CGCCATGGCCTCTGTCTCCGAGCTCGCCTGCATCTACTCGGCCCTCATTC15660.13572154722563837No Hit
GGATTTTCAGGAACAAGATCTGTTAGGGGATCCAACCACACTGTGCTTTT15650.13563487957096043No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14910.12922147312479362No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14280.12376141088008402No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC13690.11864801925408616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13520.11717466912456134No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13440.11648132788713791No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.11310128935469864Clontech SMART CDS Primer II A (100% over 21bp)
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12960.11232128046259728No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG12920.11197460984388556No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12910.11188794218920763No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG12790.11084793033307246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12510.10842123600209043No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12400.1074678918006332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11780.10209449721060153No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11640.10088115004511052No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG11630.10079448239043258No Hit
GTACAAAGAACATGCCAGTTGGAAGCTACAGCATTTAGCAGAAGTTCTAA11580.10036114411704293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)