FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94247
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1004210.654981060405106No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31843.3783568707757277No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA23422.4849597334663174No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13731.4568102963489553No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC6960.7384850446168048No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG6040.6408692053858478No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT5540.5878171188472843No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG4740.5029337803855826No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG4110.4360881513469925No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC3810.4042568994238544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.39046335692382783No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA3370.3575710632699184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.34165543730834935No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC3020.3204346026929239No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA2690.285420225577472No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC2630.27905397519284436No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC2590.2748098082697592No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2570.27268772480821674No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2500.2652604326928178No Hit
GCAATCGCAGGCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCAC2500.2652604326928178No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC2480.26313834923127527No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT2470.26207730750050395No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG2460.26101626576973275No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA2440.25889418230819017No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT2380.25252793192356254No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT2300.2440395980773924No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT2290.2429785563466211No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC2230.23661230596199348No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT2150.2281239721158233No Hit
CTGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGAT2020.2143304296157968No Hit
GTCACAGCTACTCAGGAGGCTGAGGCAGGGGAATAGCATGAACCCGGGAG1990.21114730442348298No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1920.2037200123080841No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT1800.1909875115388288RNA PCR Primer, Index 11 (95% over 23bp)
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1770.187804386346515No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC1760.18674334461574374No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA1700.18037709423111611No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG1650.17507188557725975No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC1650.17507188557725975No Hit
GTACATGGGAGGTTGAGGCCGCAGCGAGCTGTGACTGCACCACTGCACTG1580.16764459346186086No Hit
TTCCAAAGGTTAGGGTATGGAAAGAGGCATAGGGGATATGGTTTGGTATG1560.1655225100003183No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1520.16127834307723324TruSeq Adapter, Index 11 (95% over 21bp)
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC1510.16021730134646195No Hit
GTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCG1420.1506679257695205No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG1380.14642375884643544No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT1360.14430167538489289No Hit
GTACATGGGGTGTTAGCCAGGGTGGTCTCGATCTCCTGACCTTGTGATCC1350.14324063365412162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1340.14217959192335036No Hit
TTCCTGGGGTTCCCTTCTTGGGCCTGCCTCTTAAAGGCCTGAGCTAGCTG1310.13899646673103652No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1280.1358133415387227No Hit
GTGGCAGGTTCACCGAAGACTTTCCCCAAAAACAAAGCACATAGCCACCT1250.1326302163464089No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC1250.1326302163464089No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.1220197990386962Clontech SMART CDS Primer II A (100% over 21bp)
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1110.11777563211561111No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT1100.11671459038483983No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT1080.1145925069232973No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA1060.11247042346175475No Hit
AGGGTATTTGGTTTCCCGAACCAGAGCTGTCTCTTATACACATCTCCGAG1020.10822625653866967No Hit
GTGTCTGGTTTGCGGGCACAGGGTCCCTCCCCAGGTTGTCTAGGGTCAGA1020.10822625653866967No Hit
CATTAATGATCTGAAAGAAGAAGATGGGGAAAAGGGGATTCCACCACAAG1020.10822625653866967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1010.1071652148078984No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC980.10398208961558458No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACAT980.10398208961558458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)