FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195923
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1005268.405725117754237No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA158851.3282627727704877No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG117430.9819194045101567No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA70270.5875796351437342No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC67620.5654210179083436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57510.48088380271974035No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA57030.47687016639031105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.4491927992019553No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52580.4396604129195608No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT49840.41674923887240234No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC48170.4027851291429298No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT41570.3475976296132778No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT35040.2929954520483342No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC33300.2784460203541532No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC29210.24424649413047494No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC25780.2155657178597619No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG23260.19449412713025838No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC22700.18981155141259096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21570.1803627825537263No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA21070.1761819113772375No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC20280.16957613491838522No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC19600.16389015011836047No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC19480.16288674103600315No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG19220.16071268802422897No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG18790.15711713881244863No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC18680.1561973471536211No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18230.15243456309478118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17750.14842092676535196No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.14699943056534576Clontech SMART CDS Primer II A (100% over 21bp)
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG17500.14633049117710756No Hit
GTACTCAGGCAATAAGAAGTACTTCTCTGCAGGCCACAGTCACGATCTCA17440.1458287866359289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16470.13771789655354066No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16410.13721619201236201No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT15620.13061041555350972No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA15300.1279346580005569No Hit
ATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGT15130.12651316180055072No Hit
GAAATGGGGAGTTCTCTGGGCCAGGCCACATTCACATTCCCCTCCCCCTG14700.12291761258877035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.11948929822404955No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA14200.11873674141228156No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14050.11748248005933493No Hit
GCCAACAGCCCTTTTGGCCTGAATTCACCTCCTCAAGGGGAGGTCTCCAT13920.11639545355344784No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA13360.1117128778357804No Hit
GTACATGGGCGCGGATCTACCATGGGCAAACCGATTCCGAACCCGCTGCT13360.1117128778357804No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG12990.1086190331651787No Hit
TCCATGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12730.10644498015340452No Hit
AGATATGAGTTCTGCAAAAGGTGGTCCGCATCCTTGGCCCTCTGGACATT12470.10427092714163036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)