FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249390
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC843466.750974475544065No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA143431.1480002241093654No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG119880.9595082400211303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58840.4709498235138747No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC58090.4649468940843131No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA57060.4567028710010485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57050.4566228319419877No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG56780.45446177734734555No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA51140.409319748037042No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC43840.35089123492264224No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT42180.3376047511185459No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT35910.28742026108741064No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC30410.24339877860395875No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC27770.22226846701190178No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC27030.21634557664140097No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC26330.21074284250714348No Hit
ACCTGGGTGTGGGGACAGTGGTGTCAGAGCCTGGGAGGGAAGGGGACCTG23030.18432995301707233No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT22950.18368964054458575No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA20740.16600100849214416No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC20710.1657608913149617No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC20390.16319964142501542No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20230.16191901648004225Clontech SMART CDS Primer II A (100% over 21bp)
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18680.1495129623256149No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC18570.14863253267594587No Hit
GTTTGTTACCACTGTGAAATACAGTTTAGAAAGGCTTAGCCAGGCATAAG18150.14527089219539135No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18120.14503077501820888No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC17880.14310983760074916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17720.14182921265577603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17510.14014839241549876No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG17040.13638655663964014No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG16810.13454565828124126No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16590.13278479898190318No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT15530.12430065872145607No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15320.12261983848117881No Hit
GAAACTAGAGGGCAGGTCTTCATAAAAGCCCTTTGAACCCCCTTCCTGCC15310.122539799422118No Hit
AAAATACCCTTGTCCATGTTTGCCTCTGTTCCCGAGAACCACTCTTAGGA15200.12165936977244896No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14750.11805761211471197No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT14400.11525624504758322No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT14400.11525624504758322No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14210.11373550292542761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.11357542480730595No Hit
GTACCCAGCCTCCTCTCCCCACCTTGTGGGTAGAGTACAGGGCACGTGGG13690.10957347185426489No Hit
AGTTTAAGTTGGCCAAGATCACACAGCCAGTAAGGGGCAGACATTGAAAG13470.1078126125549268No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC13350.10685214384619694No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13230.10589167513746708No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA13200.10565155796028462No Hit
CAAGTGTGTCTCCTGTGCTGCGATGGGGGATCAGCAGGGCAGTTGTCGGG13070.10461105019249393No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12960.1037306205428249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)