FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976614
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10045510.286049554890674No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA175011.7920078966715614No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG114581.1732373281562622No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC60610.6206136713174294No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA58860.602694616296715No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG53970.552623656838833No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA51630.5286633204111348No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC49990.5118706059917224No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT42950.4397848075083912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.39933894046163576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37960.3886899020493255No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT34650.3547972894101457No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC30830.315682552164929No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC29520.3022688595494228No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26050.26673793330834905No Hit
TAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG25440.2604918627011286No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT23560.24124167787887538No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC23320.23878420747603454No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA22680.23223095306845898No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA22070.22598488246123855No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG21910.22434656885934462No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC19190.19649523762714846No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18570.19014677241980968No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA18180.18615338301519332No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG17320.17734744740501365No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC16960.1736612418007524No Hit
ATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAG16940.17345645260051565No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG16610.1700774307966095No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC15590.15963318158453596No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC15300.15666373818110327No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG14970.15328471637719715No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG14830.15185119197553998No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14710.15062245677411956No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14500.14847217017163383No Hit
GTACATGGGATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT14180.14519554296784606No Hit
GCACTTTGGGAGGCCAAGGCAAGTGGATCACTTGAGGTCAGGAGACCAGC14170.1450931483677277No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC14130.1446835699672542No Hit
TTACAGCAATACGGCGGCATCTTTGGTGGCTGCTGGACAGGCTCAGTACG14050.14386441316630727No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG11960.12246394174156833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.12225915254133159Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11910.12195196874097648No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG11650.11928970913789891No Hit
ATCCTGGCCAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAA11510.11785618473624175No Hit
AGAATGGGGAGCTTGAGTTCTTCTATATGCAGAAAAGAAGGAGCCAGGAA11470.11744660633576827No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11410.11683223873505807No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG10940.11201969252949476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10270.10515925432156409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.10515925432156409No Hit
GAGCTGATATCATGCCCCTGCACTCCAGCCTGGGCAACAGAGTGAGACTC10050.10290657311896No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA9970.10208741631801306No Hit
GTATCAACGCAGAGTACATGGGAGGCGGAGTTTTCAAAGCCCGCTTTGTT9950.10188262711777632No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9810.10044910271611916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)