FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073416
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA14729913.722452432235032No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA202041.882215282798095No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG165561.5423656811525075No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA83900.7816168195741445No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG79820.7436073246532565No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC50190.4675726838429835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37540.3497246174828771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.33295572266483825No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC34150.3181431989089039No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA32010.2982068461807911No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31870.2969025988060547No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA30670.2857233355940288No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA30150.2808789882021509No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC27640.25749569598366334No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG26620.24799332225344134No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT26570.24752751961960692No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25310.23578929324697975No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC23680.22060412738397786No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG23080.21501449577796491No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA22660.21110175365375586No Hit
GAAGAAGGTATCTTTGGGTCTGGCTCTTGTCTATGTTTCTTGGAACAGAA22530.2098906668057864No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT20630.1921901667200787No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA20190.18809110354233588No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA19260.1794271745530158No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC19060.17756396401767816No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC18950.17653919822324243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18000.1676889481803886No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA17790.16573257711828404No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC16200.15092005336234973No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC15280.14234928489979654No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15090.14057923489122576No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14940.13918182698972253No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG14810.13797074014175306No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14670.1366664927670167No Hit
GTGATATTTGGGTCCCCACTGACGGAGGAAGGCATTGCCCAGATATACCA13880.12930681115243298No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC13610.12679147692972714No Hit
GAGTACATGGGCAAGAAATTAAAGAAAAAGAATGGTACCCCACTGCACTT13600.12669831640296028No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT13520.12595303218882523No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT12530.11673014003890382No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12270.11430796634296489No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.11430796634296489Clontech SMART CDS Primer II A (100% over 21bp)
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11780.10974310053138764No Hit
GGGCATGGTAGCTCATTCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCA11630.1083456926298844No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11610.10815937157635065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11200.10433978997890846No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC11160.10396714787184094No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG11150.10387398734507404No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10990.10238341891680393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10860.10117233206883446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)