FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422430
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG111552.6406741945411074No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC47731.129891342944393No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA34290.8117321212982034No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25980.6150131382714296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.5688516440593707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.5679047416139952No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG21470.5082498875553346No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17760.42042468574675096No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA17610.4168738015765926No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA17520.41474327107449754No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC13040.3086901971924343No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12480.2954335629571763No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11280.26702648959590936No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA11120.2632388798144071No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT10140.24003976990270579No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT10010.23696233695523516No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC9190.2175508368250361No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG8310.19671898302677368No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT8250.19529862935871034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.19387827569064697No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC7510.17778093411926235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.17612385483985513Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7410.17541367800582344No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7060.16712828160878726No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC6730.15931633643443885No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA6730.15931633643443885No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC6200.1467698790332126No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6170.14605970219918094No Hit
GTATCAACGCAGAGTACATGGGAGGCGGAGTTTTCAAAGCCCGCTTTGTT5970.1413251899723031No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG5920.14014156191558363No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5910.13990483630423975No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC5630.1332765191866108No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG5550.13138271429585965No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5530.13090926307317188No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG5370.1271216532916696No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5300.1254645740122624No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT5300.1254645740122624No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5160.12215041545344792No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA5140.12167696423076013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5010.11859953128328954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4990.11812608006060177No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4770.11291811661103615No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC4730.11197121416566058No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT4700.11126103733162891No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT4570.10818360438415832No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA4560.10794687877281443No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC4380.10368581776862439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)