FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583569
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA193163.3099770549840724No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC47030.8059029866219761No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA45580.7810558819951026No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG35720.6120955705323621No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA23500.40269445429760664No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA21830.3740774441411384No Hit
AACCTGGAGATGGGGATCACTGCAGGAAACTGCATCACCCCCAGTATGGG19730.33809198226773524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.29045408512103965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.26012348154202847No Hit
TTTTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCCAGCTGGTCCTGAC15110.25892396614624835No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14850.2544686232476365No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12150.20820160083897532No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT10270.17598604449516683No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA9840.16861759277823188No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8680.148739909076733No Hit
ACAGTCGTCCGCCAGCGCGGCACCCACTTCCACACGGCGCAGGCGTAGGC8420.1442845661781212No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA8410.1441132068358669No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA8300.1422282540710696No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA8150.13965786393725507No Hit
TTGCAAGCAGCCTGAGCAACGCAGCCAGATCCAAATTAACAGCAAGAAAA8130.13931514525274646No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC7000.11995153957801048No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC6760.11583891536390727No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC6280.10761366693570083No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC6230.10675687022442934No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC6080.10418648009061482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)