FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677554
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC462346.823662763410739No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA125741.8557930438016748No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC91751.3541356113313479No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG80611.189720671710299No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA51220.7559545069470478No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA47370.6991324676704735No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG33570.4954586645492463No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA29830.4402601121091455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.3995253514849001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.38151940657128436No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA25740.3798959197348108No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA23650.34904966984181335No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC21880.32292629074582985No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC20050.29591737337540625No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC20030.29562219395059286No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17010.2510501008037736No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15400.22728815710629707No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC14560.2148906212641354No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT14240.21016775046712144No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12860.18980037015499873No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG12610.18611062734483155No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA12340.18212570510985102No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC12190.17991185942375074No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT11860.17504139891433007No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG10790.15924929968681462No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC10430.15393607004017393No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT10300.15201740377888698No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT10140.14965596838038003No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9370.13829156052506517No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC9230.13622530455137155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.13430663829008463No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC9080.13401145886527127No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8990.1326831514536111No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8530.1258940246829035No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.12294223043476979Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8080.11925248762460262No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7950.11733382136331569No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7830.11556274481443546No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7380.10892120775613456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)