FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925239
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCAAGTCAGAGCCTCGAA87360.45376184463331565No Hit
GGTATAGGCAGAGGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGG78630.4084168251318408No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63080.3276476323199354No Hit
GCCTATACCAATCCACATAGTTGAACCCGTTATCATTTTCGAGGCTCTGA62870.32655685865495143No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62500.3246350193404559No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT61570.3198044502526699No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA53800.27944582464826445No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAACGTGGAAATCAAACG50860.26417499333848943No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCC48880.25389055592578375No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA48570.2522803662298551No Hit
GGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGGTCCCGTCGGGCC46930.2437619433223615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46440.24121680477073235No Hit
GTAATAAACCCCAACATCTTCAGTCTCCACTCTGCTGATTTCCAGTGTAA45610.2369056517138911No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45220.23487992919320666No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43980.22843917040949202No Hit
GCCCTGGCCTCTGCCTATACCAATCCACATAGTTGAACCCGTTATCATTT42910.2228814188783834No Hit
GTCAGGGACCCCGGAGGCCCGACGGGACCCCAAGTAGATCAGGAGTTGTG41030.2131163974966225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT40490.21031155092952097No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG39890.20719505474385258No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC38340.1991441062642093No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC38300.1989363398518314No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG37330.1938980043516675No Hit
GAGTTTGTAGTGCTTGCATGCAGTAATAAACCCCAACATCTTCAGTCTCC37290.1936902379392896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36940.19187228183098307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36790.19109315778456595No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGACTCATCACAATATCC36440.1892752016762594No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG36330.1887038440422202No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGGA36310.18859996083603126No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCAA35920.18657423831534684No Hit
GGTCAAGTCAGAGCCTCGAAAATGATAACGGGTTCAACTATGTGGATTGG35750.18569123106274077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35510.18444463258847343No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35500.18439269098537897No Hit
ACTTGGGGTCCCGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT34630.1798737715161598No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAACGTGGAAATCAAACGAACTGTG34550.17945823869140404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG34400.17867911464498695No Hit
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCA33700.17504320242837385No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33550.17426407838195673No Hit
TTATTACTGCATGCAAGCACTACAAACTCCTCGGACGTTCGGCCAAGGGA33300.17296553830459493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA32770.17021263334058784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.169900983722021No Hit
GATTTTACACTGGAAATCAGCAGAGTGGAGACTGAAGATGTTGGGGTTTA31350.1628369257011727No Hit
CAGTAATAAACCCCAACATCTTCAGTCTCCACTCTGCTGATTTCCAGTGT30580.15883742226289826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.15883742226289826No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA30460.1582141230257646No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC30450.1581621814226701No Hit
TCCGAGGAGTTTGTAGTGCTTGCATGCAGTAATAAACCCCAACATCTTCA29700.15426656119058466No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACGTTGGTCCCTTGGCCGAACGTC29680.1541626779843957No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCACTGGGGA29570.1535913203503565No Hit
GACTTGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG29460.1530199627163173No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG29330.15234472187608916No Hit
AGTCAGAGCCTCGAAAATGATAACGGGTTCAACTATGTGGATTGGTATAG28810.14964375851517656No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28800.1495918169120821No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28180.14637143752022477No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT28040.14564425507690215No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC27540.1430471749221785No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA27370.14216416766957246No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26920.13982679553032118No Hit
GTTTATTACTGCATGCAAGCACTACAAACTCCTCGGACGTTCGGCCAAGG26780.13909961308699856No Hit
GGTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTC26230.13624282491680254No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG26150.13582729209204675No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT25980.1349442848394407No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25640.13317827033422863No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25510.13250302949400047No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25420.13203555506615022No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC25380.13182778865377234No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC25150.13063313178259947No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA24690.1282438180402537No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24360.12652974513813608No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG23860.12393266498341245No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT23740.12330936574627878No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC23710.12315354093699535No Hit
GTCCAGGGACCTGTCGCAGCCAACTAATGCAGTAATTTTCAAACATGTAG23530.12221859208129485No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23530.12221859208129485No Hit
CTCCACAACTCCTGATCTACTTGGGGTCCCGTCGGGCCTCCGGGGTCCCT23510.12211470887510589No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC23220.12060840238536617No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT23080.11988121994204355No Hit
GCTAATGCTCTGGGTCTCTGGGTCCACTGGGGATATTGTGATGAGTCAGT23060.11977733673585463No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23010.11951762872038224No Hit
GGGGATATTGTGATGAGTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGG22980.11936180391109884No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22930.11910209589562648No Hit
ATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC22540.11707637337494202No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22410.11640113253471387No Hit
GTTGAACCCGTTATCATTTTCGAGGCTCTGACTTGACCTGCAGGAGATGG22160.11510259245735206No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22140.11499870925116311No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA22130.11494676764806863No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21770.1130768699366676No Hit
CTCCTGATCTACTTGGGGTCCCGTCGGGCCTCCGGGGTCCCTGACAGGTT21630.11234968749334498No Hit
GTTGTGGAGACTGCCCTGGCCTCTGCCTATACCAATCCACATAGTTGAAC21410.11120697222526657No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCACTGGGGATATTG21110.1096487241324324No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21110.1096487241324324No Hit
GCCACAGTTCGTTTGATTTCCACGTTGGTCCCTTGGCCGAACGTCCGAGG20900.10855795046744847No Hit
GTATCAACGCAGAGTACATGGGGGATCAGGACTCCTCAGTTCACCTTCTC20860.10835018405507056No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20720.10762300161174794No Hit
GTACATGGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCC20630.1071555271838977No Hit
ACCCCAAGTAGATCAGGAGTTGTGGAGACTGCCCTGGCCTCTGCCTATAC20470.10632446153438613No Hit
GTCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCC20450.10622057832819717No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20340.10564922069415798No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20160.10471427183845745No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT20150.10466233023536298No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19780.1027404909208675No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA19410.10081865160637199No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA19350.10050700198780516No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19290.10019535236923832No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19260.10003952755995489No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)