FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46470
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA20144.333978911125457No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC4931.060899505057026No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG2700.5810200129115558No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA2420.5207660856466537No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT2340.5035506778566817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.4777275661717237No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT2140.46051215838175164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.43468904669679365No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT1990.4282332687755542No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT1980.42608134280180765No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1540.33139659995696147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1510.32494082203572194No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC1350.2905100064557779No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG1270.2732945986658059No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC1250.2689907467183129No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTACTCCTACCACTGTCTCT1170.25177533892834086No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA1140.24531956100710137No Hit
CGTCAGATGTGTATAAGAGACAGTCCATGAGGGATTCCTCTCTGTCTCTT1000.2151925973746503No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT1000.2151925973746503No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA950.2044329675059178No Hit
GGCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTC890.19152141166343878No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA880.18936948568969228No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA740.15924252205724124No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAG720.1549386701097482No Hit
GTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATAC720.1549386701097482No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1549386701097482Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG720.1549386701097482No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA710.1527867441360017No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT710.1527867441360017No Hit
GATCTCAGCTCACTGCAAGCTCTGCCTTCCAGGTTCAGGTCATTCTCCTG660.14202711426726922No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG630.1355713363460297No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA630.1355713363460297No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT620.1334194103722832No Hit
CGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGG620.1334194103722832No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA610.1312674843985367No Hit
CGTCAGATGTGTATAAGAGACAGGCATCAGAGGGAGACCTGTCTCTTATA600.12911555842479017No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTCCTACCACTGTCTCT590.12696363245104367No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC570.12265978050355068No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA560.12050785452980418No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC560.12050785452980418No Hit
TCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACA520.11190015063481816No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG520.11190015063481816No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG470.10114052076608565No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)