FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613158
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG41080.6699741339100199No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA28060.45763082272432226No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA27910.4551844712129663No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC16750.2731759187680826No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA16110.2627381523196305No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA15700.2560514581885909No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATACCT15280.24920167395679418No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG13910.22685833015307638No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC13080.21332185179024002TruSeq Adapter, Index 3 (95% over 22bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTG12970.21152786068191232No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCT12130.19782829221831894No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAA10670.17401713750778755No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATACCC8370.13650641433366278No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA7630.12443774687764002No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGA7520.12264375576931232No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAA7240.11807723294811451No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC6810.11106435861556074No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATC6540.10666092589512001No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAAAAAAAAAAAAAAA6510.10617165559284883No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC6470.10551929518982056No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACCTGTCTCTTA6290.10258367337619341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)